Isolation and molecular analysis of circulating tumor cells from lung cancer patients using a microfluidic chip type cell sorter

Autor: Hisao Imai, Ryo Ko, Akira Ono, Masaru Watanabe, Masahiro Endo, Toshiaki Takahashi, Tetsuhiko Taira, Haruyasu Murakami, Yasuhiro Koh, Takashi Nakajima, Masato Abe, Kazushige Wakuda, Tateaki Naito, Hirotsugu Kenmotsu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
Male
Cancer Research
Lung Neoplasms
EGFR
Microfluidics
Cell Separation
circulating tumor cell
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Circulating tumor cell
Clinical Research
Carcinoma
Non-Small-Cell Lung
Cell Line
Tumor
Biopsy
medicine
Humans
Digital polymerase chain reaction
Prospective Studies
Liquid biopsy
Lung cancer
Aged
Aged
80 and over
medicine.diagnostic_test
liquid biopsy
business.industry
Epithelial cell adhesion molecule
General Medicine
Original Articles
Middle Aged
medicine.disease
Epithelial Cell Adhesion Molecule
Neoplastic Cells
Circulating
Primary tumor
ErbB Receptors
lung cancer
030104 developmental biology
Oncology
chemistry
030220 oncology & carcinogenesis
cell sorter
Cancer research
Adenocarcinoma
Original Article
Female
business
Zdroj: Cancer Science
ISSN: 1349-7006
1347-9032
Popis: Circulating tumor cells (CTCs) are a tumor-derived material utilized for liquid-based biopsy; however, capturing rare CTCs for further molecular analysis remains technically challenging, especially in non-small-cell lung cancer. Here, we report the results of a clinical evaluation of On-chip Sort, a disposable microfluidic chip-based cell sorter, for capture and molecular analysis of CTCs from patients with lung adenocarcinoma. Peripheral blood was collected from 30 metastatic lung adenocarcinoma patients to enumerate CTCs using both On-chip Sort and CellSearch in a blind manner. Captured cells by On-chip Sort were subjected to further molecular analysis. Peripheral blood samples were also used for detection of EGFR mutations in plasma using droplet digital PCR. Significantly more CTCs were detected by On-chip Sort (22/30; median 5; range, 0-18 cells/5 mL blood) than by CellSearch (9/30; median, 0; range, 0-12 cells/7.5 mL) (P < 0.01). Thirteen of 30 patients who had a negative CTC count by CellSearch had a positive CTC count by On-chip Sort. EGFR mutations in CTCs captured by On-chip Sort were observed in 40.0% (8/20) of patients with EGFR-mutated primary tumor. EGFR mutations were often observed in 53.3% (8/15) of patients detected in plasma DNA. Expressions of EGFR and vimentin protein on CTCs were also successfully assessed using On-chip Sort. These results suggest that On-chip Sort is an efficient method to detect and capture rare CTCs from patients with lung adenocarcinoma that are undetectable with CellSearch. Mutation detection using isolated CTCs remains to be further tackled (UMIN000012488).
Databáze: OpenAIRE
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