Characterization of mitochondrial iron uptake in HepG2 cells
| Autor: | Marco T. Núñez, Z. Ioav Cabantchik, Dora Inés Mazariegos, Bruce K. Cassels |
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| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Ataxia Iron Cell Biology Mitochondrion General Biochemistry Genetics and Molecular Biology Cell Line Rhodamine chemistry.chemical_compound Extracellular medicine Humans HepG2 cells lcsh:QH301-705.5 Fluorescent Dyes Iron uptake Rhodamines General Medicine mitochondria medicine.anatomical_structure lcsh:Biology (General) Biochemistry chemistry Hepg2 cells Iron content iron homeostasis medicine.symptom General Agricultural and Biological Sciences RPA |
| Zdroj: | Biological Research, Vol 39, Iss 1, Pp 199-201 (2006) Biological Research v.39 n.1 2006 SciELO Chile CONICYT Chile instacron:CONICYT Biological Research, Volume: 39, Issue: 1, Pages: 199-201, Published: 2006 BIOLOGICAL RESEARCH Artículos CONICYT |
| ISSN: | 0716-9760 |
| Popis: | There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content. |
| Databáze: | OpenAIRE |
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