MXB inhibits murine cytomegalovirus
| Autor: | Ananda Ayyappan Jaguva Vasudevan, Dieter Häussinger, Ariane Bähr, Carsten Münk, Anna Singer, Albert Zimmermann, Ramona Grothmann |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Myxovirus Resistance Proteins Muromegalovirus Viral protein viruses GTPase Biology medicine.disease_cause Virus Replication 03 medical and health sciences stomatognathic system Virology medicine Humans Immunologic Factors Dynamin Innate immune system virus diseases Cytomegalovirus Virus Internalization 030104 developmental biology medicine.anatomical_structure HEK293 Cells Capsid Host-Pathogen Interactions Nucleus Nuclear localization sequence |
| Zdroj: | Virology. 522 |
| ISSN: | 1096-0341 |
| Popis: | The MX dynamin GTPases inhibit diverse viruses at early post-entry phases. While MXA acts antiviral against influenza viruses, the anti HIV-1 activity of MXB was discovered recently. Here, we have studied the antiviral effect of MX proteins on murine cytomegalovirus (MCMV). Our data demonstrate that human MXB but not other human or murine MX proteins inhibit MCMV propagation. Evidently, the viral protein expression was delayed and the viral DNA amount in nucleus was diminished in MXB expressing cells indicating an obstruction of nuclear entry. Of note, MCMV did not deplete MX proteins. Considering the role of capsid on HIV-1 sensitivity to MXB, MXB binding to tested MCMV capsids was not detected. Moreover, MCMV restriction occurred only when MXB contained both the nuclear localization signal and a functional GTPase domain. Hence, we propose a new mode of inhibition of MCMV by MXB that is conspicuously different from that of HIV-1. |
| Databáze: | OpenAIRE |
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