Evaluation of filters for recovery of Campylobacter jejuni from water
| Autor: | B H Keswick, J J Mathewson, Herbert L. DuPont |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Bacteriological Techniques
Chromatography Ecology biology Microporous material biology.organism_classification Applied Microbiology and Biotechnology Campylobacter jejuni Filter (aquarium) law.invention Campylobacter fetus law Evaluation Studies as Topic Depth filter Waterborne pathogen Water Microbiology Surface water Filtration Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 46(5) |
| ISSN: | 0099-2240 |
| Popis: | Campylobacter jejuni has been incriminated in several large waterborne outbreaks, but it has rarely been isolated from water itself. Better methodology is needed for the isolation of C. jejuni from water. We evaluated three types of 0.45-micron microporous filters and three different pore sizes of positively charged depth filters for their ability to recover C. jejuni from seeded, sterile tap and surface water. The microporous filters tested were Millipore HA, Gelman GN6, and Zetapor. Three pore sizes of Zeta Plus depth filters (05S, 30S, and 50S) were evaluated by using an adsorption-elution technique. The overall percent recovery in both tap and surface water by microporous filters was: Zetapor, 66%; Millipore HA, 33%; and Gelman GN6, 33%. Adsorption-elution with Zeta Plus 50S allowed 89% recovery of C. jejuni. These data suggest that both the positively charged Zetapor microporous filter and the Zeta Plus 50S depth filter are effective filters for the recovery of C. jejuni from water. |
| Databáze: | OpenAIRE |
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