High levels of ANXA2 are characteristic of malignant salivary gland tumors
| Autor: | Marcela Gonçalves de Souza, Eloá Mangabeira Santos, Luis Paulo Oliveira, Alfredo Maurício Batista de Paula, Lorena Dos Reis Pereira Queiroz, Cristiane Karine Cordeiro Santos, Erivelton Pereira dos Santos, Sérgio Henrique Sousa Santos, Claudio Marcelo Cardoso, Lucyana Conceição Farias, André Luiz Sena Guimarães, Sabrina Ferreira de Jesus |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Cancer Research Pathology medicine.medical_specialty Proteome Adenoma Pleomorphic Malignancy Pathology and Forensic Medicine Metastasis Pleomorphic adenoma 03 medical and health sciences 0302 clinical medicine medicine Biomarkers Tumor Neoplasm Humans Annexin A2 Salivary gland business.industry Not Otherwise Specified 030206 dentistry medicine.disease Salivary Gland Neoplasms Carcinoma Adenoid Cystic medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Case-Control Studies Periodontics Adenocarcinoma Immunohistochemistry Carcinoma Mucoepidermoid Oral Surgery business |
| Zdroj: | Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral PathologyREFERENCES. 48(10) |
| ISSN: | 1600-0714 |
| Popis: | Objective Malignant salivary gland tumors (MSGTs) present different phenotypic characteristics and various clinical outcomes, which proved to be a diagnostic challenge. Considering the heterogeneity of MSGT, this study aims to identify molecule related to the nature of MSGT. Methods For screening, proteomic analysis comparing MSGT with pleomorphic adenoma (PA) and salivary gland was performed. The MSGT-associated protein which presented in the higher number in the Gene Expression Omnibus (GEO) database was selected. To validate the data, immunohistochemistry (IHC) was performed in 14 patients with PA, 22 patients with MSGT, and 14 controls. Results 16 proteins were associated with MSGT. ANXA2 was the primary protein, according to GEO database analyses. ANXA2 was most expressed in the cell membrane. However, some ANXA2 staining was also observed in the cytoplasm and nucleus. ANXA2 was highly expressed in MSGT in comparison with control. Also, ANXA2 has a higher expression in adenocarcinoma not otherwise specified (ANOS) and myoepithelial carcinoma (MC) in comparison with PA. Conclusion In conclusion, this study demonstrated that MSGT presented higher levels of ANXA2 in comparison with normal salivary glands. Also, ANXA2 might be interesting as a molecular marker of ANOS and MS. |
| Databáze: | OpenAIRE |
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