Comparative analysis of the microbial communities in raw milk produced in different regions of Korea
| Autor: | Yoo Kyung Hur, Jong Geun Kim, Young-Tae Ahn, Yun-Jaie Choi, In Seon Kim, Chul-Sung Huh, Eunji Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Firmicutes 030106 microbiology Food spoilage lcsh:Animal biochemistry medicine.disease_cause Article Raw Milk Microbiota Milk Quality Psychrotrophic Bacteria Actinobacteria 03 medical and health sciences chemistry.chemical_compound fluids and secretions medicine Bulk tank Food science Lactose Dairy farming lcsh:QP501-801 lcsh:SF1-1100 biology food and beverages Raw milk biology.organism_classification Animal Biotechnology Psychrotrophic bacteria chemistry Animal Science and Zoology lcsh:Animal culture Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 30, Iss 11, Pp 1643-1650 (2017) ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(30): 11 Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | Objective: The control of psychrotrophic bacteria causing milk spoilage and illness due to toxic compounds is an important issue in the dairy industry. In South Korea, Gangwon-do province is one of the coldest terrains in which eighty percent of the area is mountainous regions, and mainly plays an important role in the agriculture and dairy industries. The purposes of this study were to analyze the indigenous microbiota of raw milk in Gangwon-do and accurately investigate a putative microbial group causing deterioration in milk quality. Methods: We collected raw milk from the bulk tank of 18 dairy farms in the Hoengseong and Pyeongchang regions of Gangwon-do. Milk components were analyzed and the number of viable bacteria was confirmed. The V3 and V4 regions of 16S rRNA gene were amplified and sequenced on an Illumina Miseq platform. Sequences were then assigned to operational taxo-nomic units, followed by the selection of representative sequences using the QIIME software package. Results: The milk samples from Pyeongchang were higher in fat, protein, lactose, total solid, and solid non-fat, and bacterial cell counts were observed only for the Hoengseong samples. The phylum Proteobacteria was detected most frequently in both the Hoengseong and Pyeongchang samples, followed by the phyla Firmicutes and Actinobacteria. Notably, Corynebacterium, Pediococcus, Macrococcus, and Acinetobacter were significantly different from two regions. Conclusion: Although the predominant phylum in raw milk is same, the abundances of major genera in milk samples were different between Hoengseong and Pyeongchang. We assumed that these differences are caused by regional dissimilar farming environments such as soil, forage, and dairy farming equipment so that the quality of milk raw milk from Pyeongchang is higher than that of Hoengseong. These results could provide the crucial information for identifying the microbiota in raw milk of South Korea. |
| Databáze: | OpenAIRE |
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