Plasma Antibody Profiles as Diagnostic Biomarkers for Tuberculosis▿
| Autor: | Kathy DeRiemer, Syed Kumail Ali Rizvi, Celia W. Goulding, Imran Khan, Phil Felgner, Sabira Tahseen, Azra Khanum, Muhammad Arif Nadeem Saqib, Viswanathan V Krishnan, Mirza Imran Shahzad, Irum Nawaz Awan, Resmi Ravindran, Paul A. Luciw, Greg C. Ireton |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Tuberculosis Clinical Biochemistry Immunology Disease cluster Sensitivity and Specificity Mycobacterium tuberculosis Plasma Tuberculosis diagnosis medicine Immunology and Allergy Clinical Laboratory Immunology Humans Multiplex Pakistan Immunoassay biology medicine.diagnostic_test business.industry Clinical Laboratory Techniques Sputum medicine.disease biology.organism_classification Antibodies Bacterial Microspheres biology.protein medicine.symptom Antibody business Biomarkers |
| Popis: | Two billion people are infected with Mycobacterium tuberculosis , the etiological agent of tuberculosis (TB), worldwide. Ten million to 20 million of the infected individuals develop disease per year. TB is a treatable disease, provided that it is diagnosed in a timely manner. The current TB diagnostic methods are subjective, inefficient, or not cost-effective. Antibody-based blood tests can be used efficiently and cost-effectively for TB diagnosis. A major challenge is that different TB patients generate antibodies against different antigens. Therefore, a multiplex immunoassay approach is needed. We have developed a multiplex panel of 28 M. tuberculosis antigen-coated microbeads. Plasma samples were obtained from over 300 pulmonary TB patients and healthy controls in a country where TB is endemic, Pakistan. Multiplex data were analyzed using computational tools by multivariate statistics, classification algorithms, and cluster analysis. The results of antibody profile-based detection, using 16 selected antigens, closely correlated with those of the sputum-based diagnostic methods (smear microscopy and culture) practiced in countries where TB is endemic. Multiplex microbead immunoassay had a sensitivity and specificity of approximately 90% and 80%, respectively. These antibody profiles could potentially be useful for the diagnosis of nonpulmonary TB, which accounts for approximately 20% of cases of disease. Since an automated, high-throughput version of this multiplex microbead immunoassay could analyze thousands of samples per day, it may be useful for the diagnosis of TB in millions of patients worldwide. |
| Databáze: | OpenAIRE |
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