Low Temperature Storage of Larvae and Synchronization of Adult Emergence in the Predatory Midge Aphidoletes aphidimyza
| Autor: | Jan Havelka, Vladimir Kostal |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Aphidoletes aphidimyza Cold storage Diapause medicine.disease_cause General Biochemistry Genetics and Molecular Biology Animal science medicine Animals Hexanes Adult stage Desiccation Pest Control Biological Cryopreservation Larva biology Ecology Diptera Temperature Environmental factor General Medicine biology.organism_classification Cecidomyiidae Aphids Predatory Behavior Midge General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 42:112-120 |
| ISSN: | 0011-2240 |
| Popis: | Diapause larvae of Aphidoletes aphidimyza were stored at a temperature of 3 degrees C under continuous darkness for up to 7 months with survival rates above 50%; after storage for 1 year the survival rate dropped to 12%. Diapause was terminated in the majority of individuals within 120 days of chilling under storage conditions. Brief exposure (10-60 s) to the vapor of n-hexane appeared to be a useful alternative to chilling for the termination of diapause. The larvae with terminated diapause required, on average, an additional 31 days at 22 degrees C and long-day conditions in order to reach the adult stage. The 10-90% adult emergence spanned a period of 21.1 days. When the larvae with terminated diapause were exposed to 30 degrees C for 1 week after the end of low temperature storage, the survival rate was not affected, the average "time-to-adult" shortened moderately to 28 days, and the synchrony of adult emergence improved considerably to 10 days. Low temperature storage of nondiapause larvae resulted in a decrease in survival from 98 to 31% during the first 60 days of storage. Nondiapause larvae did not enter diapause during low temperature storage and, as a consequence, the adults emerged relatively rapidly (after 14-15 days) and synchronously (within 2-3 days) after the end of storage. Directions for future research, which might bring further improvement in low temperature storability and synchrony of adult emergence in A. aphidimyza, are proposed. |
| Databáze: | OpenAIRE |
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