In Vitro and In Vivo Studies Demonstrate Anticancer Property of Root Extract of Polygala senega
| Autor: | Soumya Sundar Bhattacharyya, Naoual Boujedaini, Saili Paul, Sushil Kumar Mandal, Anisur Rahman Khuda-Bukhsh |
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| Rok vydání: | 2010 |
| Předmět: |
Lung Neoplasms
mice Polygala Cell Survival Polygala senega Gene Expression Pharmacology Plant Roots In vivo Cell Line Tumor Animals Viability assay A549 cell anticancer potentials biology Plant Extracts Chemistry A549 adenoma cell line General Medicine Aryl hydrocarbon receptor biology.organism_classification Antineoplastic Agents Phytogenic In vitro Proliferating cell nuclear antigen lung cancer Anesthesiology and Pain Medicine Complementary and alternative medicine Apoptosis Immunology biology.protein Signal Transduction |
| Zdroj: | Journal of Acupuncture and Meridian Studies. 3(3):188-196 |
| ISSN: | 2005-2901 |
| DOI: | 10.1016/s2005-2901(10)60035-0 |
| Popis: | Polygala senega is extensively used in traditional systems of medicine against various lung diseases including cancer. In the present study we tested the anticancer potentials of ethanolic extract of roots of P. senega (generally used as a homeopathic drug) in a mammalian model, where mice, in vivo, were treated chronically with benzo[a] pyrene and in vitro where lung adenocarcinoma cell line (A549) were used. We deployed various parameters like cell viability assay, chromatin condensation studies with Hoechst 333258 staining, and maintained suitable controls. To understand the possible signal transduction pathways, expression of various signal proteins such as Aryl Hydrocarbon receptor (AhR), cytochrome P450 (CYP1A1), Bcl-2, proliferating cell nuclear antigen (PCNA), Bax and Caspase-3 was studied. Additionally, reverse transcriptase polymerase chain reaction analysis of AhR, p53, PCNA and β-actin (housekeeping) genes was made. Immunohistochemical localization of PCNA proteins was also conducted in vivo. Feeding of root extract of P. senega to mice (at the rate of 50 mg/kg and 100 mg/kg bw) chronically treated with the carcinogen (50 mg/kg bw dissolved in olive oil) showed positive modulation in expression of signal proteins. Upregulation of apoptotic signals such as p53, Caspase-3 and Bax, and downregulation of AhR, cytochrome P450 (CYP1A1), Bcl-2 and PCNA were observed. Addition of root extract of Polygala Senega (at doses of 50 μg and 100 μg) into culture medium containing A549 cells induced recovery of decreased cell viability and increased chromatin fragmentation (apoptosis). Therefore, results of both in vivo and in vitro studies scientifically validate its potential use as an anticancer agent, particularly against lung cancer, and provide important information potentially helpful in drug designing. |
| Databáze: | OpenAIRE |
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