Cell penetrating, mitochondria targeting multiply charged DABCO-cyanine dyes
| Autor: | Lucija Horvat, Ivo Crnolatac, Iva Orehovec, Atanas Kurutos, Dijana Saftić, Ivo Piantanida, Todor Deligeorgiev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
010405 organic chemistry Process Chemistry and Technology General Chemical Engineering Organic Chemistry Cationic polymerization Isothermal titration calorimetry DABCO 010402 general chemistry 01 natural sciences Fluorescence Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound Chemistry chemistry MTT assay Cyanine cyanine mitochondria fluorescence confocal microscopy Alkyl Methyl group |
| Zdroj: | Dyes and Pigments |
| Popis: | We have prepared new series of lipophilic cyanine dyes equipped with several cationic quaternary ammonium moieties. This was achieved by introducing DABCO within the structures. Furthermore, to investigate the influence of the additional cationic charges on the cellular uptake, DABCO was quaternized with methyl group or alkyl piperidine cation. Spectroscopic and isothermal titration calorimetry studies as well as MTT assay and subcellular localization using confocal laser scanning microscopy revealed that some of the presented dyes combine low anti-proliferative effect with efficient cellular uptake, high affinity towards ds-DNA/RNA and remarkable fluorescent marking of mitochondria. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|