Dendritic Cells Are Involved in the Effects of Exercise in a Model of Asthma
| Autor: | Adilson Santos Andrade-Sousa, Tobias Müller, Rodolfo de Paula Vieira, Manoel Carneiro Oliveira-Junior, Sanja Cicko, Adriano Silva-Renno, Maysa Alves Rodrigues Brandão-Rangel, Melanie Grimm, Alana Santos-Dias, BreAnne MacKenzie, Milton A. Martins, Erasmo Assumpção-Neto, Marco Idzko, Ana Paula Ligeiro Oliveira |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine T-Lymphocytes Physical Therapy Sports Therapy and Rehabilitation Inflammation Lymphocyte Activation 03 medical and health sciences 0302 clinical medicine Physical Conditioning Animal medicine Animals Aerobic exercise Orthopedics and Sports Medicine Asthma Physical conditioning biology business.industry Dendritic Cells respiratory system medicine.disease Mice Inbred C57BL Disease Models Animal Ovalbumin 030104 developmental biology 030228 respiratory system Immunology Lymphocyte activation biology.protein Airway Remodeling Cytokines medicine.symptom Airway business Bronchoalveolar Lavage Fluid |
| Zdroj: | Medicine & Science in Sports & Exercise. 48:1459-1467 |
| ISSN: | 0195-9131 |
| Popis: | This study investigated the effects of aerobic exercise (AE) on both the maturation of dendritic cells (DC) and the activation of lymphocytes in a mouse model of chronic allergic airway inflammation.C57BL/6 mice distributed into control, exercise, ovalbumin (OVA), and OVA + exercise groups were submitted to OVA sensitization and challenge. Treadmill training was performed for 4 wk, and mice were assessed for classical features of chronic allergic airway inflammation as well as dendritic cell activation and T-lymphocyte response.AE reduced OVA-induced eosinophilic inflammation as observed in bronchoalveolar lavage fluid (P0.001), airway walls (P0001), and also reduced collagen deposition (P0.001). AE also reduced bronchoalveolar lavage fluid cytokines (interleukin [IL]-4, P0.001; IL-5, P0.01; IL-6, P0.001; IL-13, P0.01; and tumor necrosis factor α, P0.01). Cells derived from mediastinal lymphnodes of AE animals that were restimulated with OVA produced less IL-4 (P0.01), IL-5 (P0.01), and IL-13 (P0.001). In addition, AE reduced both DC activation, as demonstrated by reduced release of IL-6 (P0.001), CXCL1/KC (P0.01), IL-12p70 (P0.01), and tumor necrosis factor α (P0.05) and DC maturation, as demonstrated by lower MCH-II expression (P0.001).AE attenuated dendritic cell and lymphocyte activation and maturation, which contributed to reduced airway inflammation and remodeling in the OVA model of chronic allergic airway inflammation. |
| Databáze: | OpenAIRE |
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