Reference intervals for C-peptide and insulin derived from a general adult Danish population
| Autor: | Allan Linneberg, Lennart Friis-Hansen, Torben Hansen, Pia Bükmann Larsen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male medicine.medical_specialty Danish population medicine.medical_treatment Denmark Clinical Biochemistry 030209 endocrinology & metabolism 030204 cardiovascular system & hematology Reference laboratory World health Fasting insulin Prediabetic State 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Diabetes mellitus medicine Humans Insulin Aged Immunoassay C-Peptide C-peptide business.industry General Medicine Fasting Middle Aged medicine.disease Reference intervals Endocrinology chemistry Female business Follow-Up Studies |
| Zdroj: | Larsen, P B, Linneberg, A R, Hansen, T & Friis-Hansen, L J 2017, ' Reference intervals for C-peptide and insulin derived from a general adult Danish population ', Clinical Biochemistry, vol. 50, no. 7-8, pp. 408-413 . https://doi.org/10.1016/j.clinbiochem.2016.12.004 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2016.12.004 |
| Popis: | Background Despite international efforts to standardize C-peptide and insulin calibrators and immunoassays, platform dependent differences still exist, and platform specific reference intervals are hence needed for correct interpretation. We therefore wanted to establish traceable reference intervals for C-peptide and insulin. Methods In 623 consecutively recruited participants, insulin and C-peptide were measured using the Cobas e411 (Roche Diagnostics, Switzerland). Participants with diabetes were excluded (fasting Glucose ≥ 7.0 mmol/L or HbA1c ≥ 6.5%/ ≥ 48 mmol/L) and reference intervals were calculated with and without the inclusion of persons who were prediabetic, according to two definitions (The World Health Organization (WHO) and American Diabetes Association (ADA)). To ensure the correctness of calibration, the control pools were analyzed by a reference laboratory. The reference intervals were calculated according to the IFCC guidelines, using the RefVal software (Solberg, Oslo, Norway). Results Comparison of our results with those from the reference laboratory revealed equivalence for C-peptide results whereas the insulin determined on the Cobas e411 assay were 15–20% higher. The difference is attributed to an incorrect conversion factor for converting from activity to metric units. The Cobas e411 assay uses the factor 6.945 for converting from U/mL to pmol/L. This is in disagreement with the biological activity of insulin which is 166.8 × 106 IU/mol or 6.00 nmol/IU. Conclusion We successfully established reference intervals for C-peptide and insulin for non-diabetic and prediabetic participants. The reference intervals for fasting C-peptide and fasting insulin are ready for implementation. A recertification of the insulin standards is needed. |
| Databáze: | OpenAIRE |
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