Near-infrared laser mediated modulation of ice crystallization by two-dimensional nanosheets enables high-survival recovery of biological cells from cryogenic temperatures
| Autor: | S. M. Chapal Hossain, Zhongrong Chen, Fazil Panhwar, Pengpeng Chen, Meng Wang, Gang Zhao, Zeeshan Haider, Kashan Memon |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
02 engineering and technology 010402 general chemistry 01 natural sciences law.invention chemistry.chemical_compound law Freezing Human Umbilical Vein Endothelial Cells Humans General Materials Science Disulfides Crystallization Molybdenum disulfide Cryopreservation Molybdenum Ice crystals Graphene Ice Biomaterial Photothermal therapy 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Chemical engineering chemistry Ice nucleus Graphite 0210 nano-technology Microfabrication |
| Zdroj: | Nanoscale. 10:11760-11774 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | Two-dimensional (2D) graphene oxide (GO) and molybdenum disulfide (MoS2) nanosheets (NSs) have been widely used as photothermal agents and as potential carriers of antitumor drugs. Their spatial thermal effects have been extensively explored for use at physiological and hyperthermic temperatures (37 to 46 °C). Furthermore, the modulation of the spatial thermal distributions with these NSs may have even more profound applications in the microstructural control of biomaterials at cryogenic temperatures (-196 to 37 °C). These applications include bioinspired microfabrication via freezing, food and drug freeze-drying, and biomaterial cryopreservation. However, such thermal effects of NSs and their applications at cryogenic temperatures had never been fully explored. Therefore, in this study, we have utilized the near-infrared laser induced photothermal effects of GO and MoS2 NSs to suppress the ice nucleation and ice crystal growth during warming of the biosamples. Using this approach, biological cells subjected to fast cooling to a deeply frozen state (-196 °C) were successfully recovered with high survival rates and full biological functionality. Thus, we provide a NS based effective approach to control the crystallization behaviors of water during warming at cryogenic temperatures, as NSs may have wide applications in both materials science and bioengineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|