Molecular typing for detection of high-risk human papillomavirus is a useful tool for distinguishing primary bladder carcinoma from secondary involvement of uterine cervical carcinoma in the urinary bladder
| Autor: | Chin Chen Pan, Chiung Ru Lai, Hua Lin Kao, Hsiang Ling Ho |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology Histology Urology Uterine Cervical Neoplasms Chromogenic in situ hybridization urologic and male genital diseases Pathology and Forensic Medicine Metastasis 03 medical and health sciences Molecular typing 0302 clinical medicine Cervical carcinoma medicine Carcinoma Humans Human papillomavirus Papillomaviridae In Situ Hybridization Bladder cancer Urinary bladder business.industry Papillomavirus Infections General Medicine medicine.disease female genital diseases and pregnancy complications Molecular Typing 030104 developmental biology medicine.anatomical_structure Urinary Bladder Neoplasms 030220 oncology & carcinogenesis DNA Viral Female business Multiplex Polymerase Chain Reaction |
| Zdroj: | Histopathology. 68:513-519 |
| ISSN: | 0309-0167 |
| DOI: | 10.1111/his.12769 |
| Popis: | Aims For patients with carcinoma of the urinary bladder and uterine cervix, distinguishing between metastasis and a second primary carcinoma has significant prognostic and therapeutic implications. The aim of this study was to investigate the prevalence of high-risk human papillomavirus (HR-HPV) in cervical carcinoma with secondary involvement of the bladder and primary bladder carcinoma, in order to explore whether the detection of HR-HPV could help to differentiate between the two. Methods and results Paired bladder and cervix carcinoma specimens from 37 patients with cervical carcinoma with bladder involvement, four patients with bladder carcinoma with uterine cervical involvement and two patients with double primaries were studied with quantitative multiplex polymerase chain reaction and chromogenic in-situ hybridization. Three hundred and seventy-five bladder carcinomas and 220 cervical carcinomas were analysed as controls. All cases of cervical carcinoma with bladder involvement showed concordant HR-HPV-positive patterns. The four cases of bladder carcinoma with uterine involvement were negative for HR-HPV. HR-HPV was detected in the cervical carcinoma but not in the bladder carcinoma of the patients with double primaries. HR-HPV was detected in 91.9% of cervical carcinomas but in none of the bladder carcinomas in the control group. Conclusions Molecular typing for HR-HPV detection is useful to distinguish bladder carcinoma from secondary involvement of cervical carcinoma. |
| Databáze: | OpenAIRE |
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