The effect of a short-term hypocaloric diet on liver gene expression and metabolic risk factors in obese women
| Autor: | Maarit Jokela, Jaana Rysä, Y. Kesäniemi, Olavi Ukkola, Maire Rantala, Mika Ilves, M. Hietaniemi, J.T. Vuoristo |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Medicine (miscellaneous) Adipose tissue Down-Regulation Gene Expression Biology chemistry.chemical_compound High-density lipoprotein Weight loss Risk Factors Internal medicine Gene expression Weight Loss medicine Humans Insulin Obesity Diet Fat-Restricted Triglycerides Aged Oligonucleotide Array Sequence Analysis Nutrition and Dietetics Triglyceride Fasting Middle Aged medicine.disease Microarray Analysis Up-Regulation Endocrinology chemistry Liver Female medicine.symptom Cardiology and Cardiovascular Medicine Body mass index |
| Zdroj: | Nutrition, metabolism, and cardiovascular diseases : NMCD. 19(3) |
| ISSN: | 1590-3729 |
| Popis: | Background and aims Most gene expression studies examining the effect of obesity and weight loss have been performed using adipose tissue. However, the liver also plays a central role in maintaining energy balance. We wanted to study the effects of a hypocaloric diet on overall hepatic gene expression and metabolic risk factors. Methods and results The study subjects were middle-aged, obese women. The diet intervention subjects ( n =12) were on a hypocaloric, low-fat diet for 8weeks with a daily energy intake of 5.0MJ (1200kcal), while the control subjects ( n =19) maintained their weight. Liver biopsies were taken at the end of the diet period during a gallbladder operation. Hepatic gene expression was analyzed using microarrays by comparing the gene expression profiles from four subjects per group. A global decrease in gene expression was observed with 142 down-regulated genes and only one up-regulated gene in the diet intervention group. The diet resulted in a mean weight loss of 5% of body weight. Triglyceride and fasting insulin concentrations decreased significantly after the diet. Conclusions The global decrease in hepatic gene expression was unexpected but the results are interesting, since they included several genes not previously linked to weight reduction. However, since the comparison was made only after the weight reduction, other factors in addition to weight loss may also have been involved in the differences in gene expression between the groups. The decrease in triglyceride and fasting plasma insulin concentrations is in accordance with results from previous weight-loss studies. |
| Databáze: | OpenAIRE |
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