Transcriptional Profiling of the Chicken Tracheal Response to Virulent Mycoplasma gallisepticum Strain R low
| Autor: | Steven J. Geary, Lawrence K. Silbart, K. Pflaum, J. Beaudet, Steven M. Szczepanek, X. Liao, Edan R. Tulman, Gerald F. Kutish |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mycoplasma gallisepticum Chemokine 030106 microbiology Immunology Inflammation Respiratory Mucosa Real-Time Polymerase Chain Reaction medicine.disease_cause Microbiology 03 medical and health sciences Immune system Gene expression medicine Animals Mycoplasma Infections Poultry Diseases Host Response and Inflammation biology Sequence Analysis RNA Gene Expression Profiling Toll-Like Receptors Immune dysregulation biology.organism_classification Toll-Like Receptor 4 Trachea 030104 developmental biology Infectious Diseases TLR4 biology.protein Cytokines Parasitology Chemokines Signal transduction medicine.symptom Chickens Metabolic Networks and Pathways Signal Transduction |
| Zdroj: | Infection and Immunity. 85 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00343-17 |
| Popis: | Mycoplasma gallisepticum , the primary etiologic agent of chronic respiratory disease (CRD) in poultry, leads to prolonged recruitment and activation of inflammatory cells in the respiratory mucosa. This is consistent with the current model of immune dysregulation that ostensibly allows the organism to evade clearance mechanisms and establish chronic infection. To date, studies using quantitative reverse transcription-PCR (qRT-PCR) and microarrays have shown a significant transient upregulation of cytokines and chemokines from tracheal epithelial cells (TECs) in vitro and tracheal tissue ex vivo in response to virulent strain R low that contributes to the infiltration of inflammatory cells into the tracheal mucosa. To expand upon these experiments, RNA was isolated from tracheas of 20 chickens infected with M. gallisepticum R low and 20 mock-infected animals at days 1, 3, 5, and 7 postinoculation, and samples were analyzed for differential gene expression using Illumina RNA sequencing. A rapid host response was observed 24 h postinfection, with over 2,500 significantly differentially expressed genes on day 3, the peak of infection. Many of these genes have immune-related functions involved in signaling pathways, including Toll-like receptor (TLR), mitogen-activated protein kinase, Jak-STAT, and the nucleotide oligomerization domain-like receptor pathways. Of interest was the increased expression of numerous cell surface receptors, including TLR4 and TLR15, which may contribute to the production of cytokines. Metabolic pathways were also activated on days 1 and 3 postinfection, ostensibly due to epithelial cell distress that occurs upon infection. Early perturbations in tissue-wide gene expression, as observed here, may underpin a profound immune dysregulation, setting the stage for disease manifestations characteristic of M. gallisepticum infection. |
| Databáze: | OpenAIRE |
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