Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia
| Autor: | Nora Cardona-Castro, Malcolm S. Duthie, Héctor Alejandro Serrano-Coll, Juan Camilo Beltrán-Alzate, Mónica Muñoz |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Tuberculosis Adolescent 030106 microbiology 030231 tropical medicine Enzyme-Linked Immunosorbent Assay Colombia Disaccharides Sensitivity and Specificity Young Adult 03 medical and health sciences 0302 clinical medicine Glycolipid Antigen Leprosy Virology medicine Humans Child Aged Aged 80 and over chemistry.chemical_classification Antigens Bacterial biology business.industry Leishmaniasis Articles Middle Aged medicine.disease Infectious Diseases Enzyme Immunoglobulin M chemistry Child Preschool Immunology biology.protein Female Parasitology Antibody Protein A business |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 98:274-277 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.17-0500 |
| Popis: | Leprosy is a chronic infectious disease with a broad spectrum of manifestations. Delays in attaining correct diagnosis permit progressive peripheral nerve damage that can produce irreversible disabilities. Tests detecting antigen-specific antibodies can aid the diagnostic process and potentially detect patients earlier. Reported tests have lacked optimal sensitivity and specificity; however, the need to develop new tests to aid early diagnosis still remains. In this study, we determined the sensitivity, specificity, positive predictive value, and negative predictive value of enzyme-linked immunosorbent assay (ELISA) using natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID). Serum samples from confirmed multibacillary patients (N = 338) and paucibacillary patients (N = 58) were evaluated and contrasted against samples from individuals without leprosy (100 healthy persons, 36 leishmaniasis or tuberculosis patients). ELISA detecting either antigen-specific IgM, IgG, or the combination of IgG and IgM (with protein A) were conducted. At a sensitivity of 78% among all patients, serum IgM antibodies against the NDO-LID conjugate were detected at a greater level than those recognizing phenolic glycolipid-I antigen (64% overall sensitivity), while providing similar specificity (97% versus 100%, respectively). Given the inclusion of the LID-1 protein within NDO-LID, we also detected conjugate-specific IgG within patient sera at a sensitivity of 81.6%. The use of protein A to simultaneously detect both antigen-specific IgG and IgM isotypes yielded the highest overall sensitivity of 86.3%. Taken together, our data indicate that the detection of both IgG and IgM antibodies against NDO-LID with protein A provided the best overall ability to detect Colombian leprosy patients. |
| Databáze: | OpenAIRE |
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