Neurotoxicity mediated by oxidative stress caused by titanium dioxide nanoparticles in human neuroblastoma (SH-SY5Y) cells
| Autor: | Daniel Gustavo Olmedo, Mariela Gisele Domingo, Analía Etcheverrito, Deborah Ruth Tasat, Sebastián Ariel Ferraro |
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| Rok vydání: | 2019 |
| Předmět: |
SH-SY5Y
Cell Survival Apoptosis 010501 environmental sciences medicine.disease_cause 01 natural sciences Biochemistry Inorganic Chemistry 03 medical and health sciences Neuroblastoma 0302 clinical medicine Cell Line Tumor medicine Autophagy Humans 0105 earth and related environmental sciences chemistry.chemical_classification Titanium Reactive oxygen species Endoplasmic reticulum technology industry and agriculture Neurotoxicity respiratory system medicine.disease Endoplasmic Reticulum Stress Cell biology Oxidative Stress chemistry Unfolded protein response Molecular Medicine Nanoparticles Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 57 |
| ISSN: | 1878-3252 |
| Popis: | Background Titanium is widely used in biomedicine. Due to biotribocorrosion, titanium dioxide (TiO2) nanoparticles (NPs) can be released from the titanium implant surface, enter the systemic circulation, and migrate to various organs and tissues including the brain. A previous study showed that 5 nm TiO2 NPs reached the highest concentration in the brain. Even though TiO2 NPs are believed to possess low toxicity, little is known about their neurotoxic effects. The aim of the study was to evaluate in vitro the effects of 5 nm TiO2 NPs on a human neuroblastoma (SH-SY5Y) cell line. Methods Cell cultures were divided into non-exposed and exposed to TiO2 NPs for 24 h. The following were evaluated: reactive oxygen species (ROS) generation, apoptosis, cellular antioxidant response, endoplasmic reticulum stress and autophagy. Results Exposure to TiO2 NPs induced ROS generation in a dose dependent manner, with values reaching up to 10 fold those of controls (p Conclusions Our results show that TiO2 NPs cause ROS increase, induction of ER stress, Nrf2 cytoplasmic translocation to the nucleus and apoptosis. Thus, neuroblastoma cell response to TiO2 NPs may be associated with an imbalance of the oxidative metabolism where endoplasmic reticulum-mediated signal pathway seems to be the main neurotoxic mechanism. |
| Databáze: | OpenAIRE |
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