The orphan pentameric ligand-gated ion channelpHCl-2is gated by pH and regulates fluid secretion inDrosophilaMalpighian tubules
| Autor: | Laura A. Nilson, Michael P. O'Donnell, Joseph A. Dent, Tanja Starc, Daniel Feingold |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Malpighian tubule system Physiology Protein subunit Malpighian Tubules Aquatic Science Biology Neurotransmission Models Biological 03 medical and health sciences Chloride Channels Neurotransmitter receptor Cyclic AMP Animals Drosophila Proteins Amino Acid Sequence Molecular Biology Ecology Evolution Behavior and Systematics Ion channel Epithelial Cells Hydrogen-Ion Concentration Ligand-Gated Ion Channels Apical membrane Body Fluids Diuresis Cell biology Protein Subunits Drosophila melanogaster 030104 developmental biology Insect Science Mutation Ligand-gated ion channel Animal Science and Zoology Homeostasis |
| Zdroj: | Journal of Experimental Biology. |
| ISSN: | 1477-9145 0022-0949 |
| DOI: | 10.1242/jeb.141069 |
| Popis: | Pentameric ligand-gated ion channels (pLGICs) constitute a large protein superfamily in metazoa whose role as neurotransmitter receptors mediating rapid, ionotropic synaptic transmission has been extensively studied. Although the vast majority of pLGICs appear to be neurotransmitter receptors, the identification of pLGICs in non-neuronal tissues and homologous pLGIC-like proteins in prokaryotes points to biological functions, possibly ancestral, that are independent of neuronal signalling. Here, we report the molecular and physiological characterization of a highly divergent, orphan pLGIC subunit encoded by the pHCl-2 (CG11340) gene, in Drosophila melanogaster We show that pHCl-2 forms a channel that is insensitive to a wide array of neurotransmitters, but is instead gated by changes in extracellular pH. pHCl-2 is expressed in the Malpighian tubules, which are non-innervated renal-type secretory tissues. We demonstrate that pHCl-2 is localized to the apical membrane of the epithelial principal cells of the tubules and that loss of pHCl-2 reduces urine production during diuresis. Our data implicate pHCl-2 as an important source of chloride conductance required for proper urine production, highlighting a novel role for pLGICs in epithelial tissues regulating fluid secretion and osmotic homeostasis. |
| Databáze: | OpenAIRE |
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