Recellularization of auricular cartilage via elastase-generated channels
| Autor: | Nicole Kops, D.M.H.J. ten Berge, Sylvia Nürnberger, B.C.J. van der Eerden, Roberto Narcisi, Wendy Koevoet, Johannes Lehmann, G.J.V.M. van Osch, Kathryn S. Stok |
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| Přispěvatelé: | Cell biology, Otorhinolaryngology and Head and Neck Surgery, Orthopedics and Sports Medicine, Internal Medicine |
| Rok vydání: | 2019 |
| Předmět: |
Adolescent
0206 medical engineering Biomedical Engineering Mice Nude Bioengineering 02 engineering and technology Biochemistry Biomaterials Extracellular matrix Ear Cartilage medicine Animals Humans Child Aged Glycosaminoglycans Decellularization Pancreatic Elastase Tissue Scaffolds biology Chemistry Cartilage Mesenchymal stem cell General Medicine Middle Aged 021001 nanoscience & nanotechnology Chondrogenesis 020601 biomedical engineering Elastin Extracellular Matrix Cell biology medicine.anatomical_structure biology.protein Cattle Female Stem cell 0210 nano-technology Biotechnology |
| Zdroj: | Biofabrication, 11(3):035012. IOP Publishing Ltd. |
| ISSN: | 1758-5090 1758-5082 |
| Popis: | Decellularized tissue matrices are promising substrates for tissue generation by stem cells to replace poorly regenerating tissues such as cartilage. However, the dense matrix of decellularized cartilage impedes colonisation by stem cells. Here, we show that digestion of elastin fibre bundles traversing auricular cartilage creates channels through which cells can migrate into the matrix. Human chondrocytes and bone marrow-derived mesenchymal stromal cells efficiently colonise elastin-treated scaffolds through these channels, restoring a glycosaminoglycan-rich matrix and improving mechanical properties while maintaining size and shape of the restored tissue. The scaffolds are also rapidly colonised by endogenous cartilage-forming cells in a subcutaneously implanted osteochondral biopsy model. Creating channels for cells in tissue matrices may be a broadly applicable strategy for recellularization and restoration of tissue function. |
| Databáze: | OpenAIRE |
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