DNA damage induced by shikonin in the presence of Cu(II) ions: potential mechanism of its activity to apoptotic cell death
| Autor: | Zhen Wu, Ying-Kun Qiu, Hong-Ming Cheng, Yufen Zhao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
DNA damage
Pharmaceutical Science Apoptosis medicine.disease_cause Analytical Chemistry Superoxide dismutase Neocuproine chemistry.chemical_compound Drug Discovery medicine Humans Pharmacology chemistry.chemical_classification Reactive oxygen species Molecular Structure biology Chemistry Organic Chemistry General Medicine Complementary and alternative medicine Biochemistry Catalase biology.protein Biophysics Molecular Medicine Sodium azide Reactive Oxygen Species Oxidation-Reduction Copper Oxidative stress DNA Damage Drugs Chinese Herbal HeLa Cells Naphthoquinones Plasmids |
| Zdroj: | Journal of Asian Natural Products Research. 13:12-19 |
| ISSN: | 1477-2213 1028-6020 |
| DOI: | 10.1080/10286020.2010.537262 |
| Popis: | Shikonin (β-alkannin), a naphthazarin derivative, has shown a variety of abilities such as anti-inflammatory, antitumoral, cytotoxic, and antimicrobial activities. In the presence of Cu(II), shikonin caused breakage of supercoiled plasmid pBR322 DNA. Other metal ions tested [Mg(II), Ca(II), and Ni(II)] were ineffective and only Fe(II) has the same ability in the DNA breakage reaction. The involvement of active oxygen in the reaction was established by the inhibition of DNA breakage by superoxide dismutase, catalase, thiourea, sodium azide, potassium iodide, and sodium benzoate. Cu(I) was shown to be an essential intermediate using the Cu(I)-specific sequestering reagent neocuproine. Shikonin induced HeLa cell apoptosis involved in the mechanism of increasing intracellular reactive oxygen species (ROS). It was suggested that shikonin generated ROS as a pro-oxidant in the presence of Cu(II), and ROS resulted in DNA damage and apoptotic cell death in cells. |
| Databáze: | OpenAIRE |
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