Assay of serum hyaluronic acid in clinical application
| Autor: | M.Mitsuo Yokoyama, Shigeki Shichijo, Takashi Matsuura, Kenji Chichibu |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Adult
Statistics as Topic Clinical Biochemistry Biotin Hyaluronoglucosaminidase Osteoarthritis Serum Hyaluronic Acid Biochemistry Arthritis Rheumatoid chemistry.chemical_compound Hyaluronidase Hyaluronic acid medicine Humans Rheumatoid factor Hyaluronic Acid Bradford protein assay Aged Aged 80 and over business.industry Hydrolysis Biochemistry (medical) General Medicine Middle Aged medicine.disease Molecular biology Hyaluronan Receptors chemistry Rheumatoid arthritis Carrier Proteins business Quantitative analysis (chemistry) Biomarkers medicine.drug |
| Zdroj: | Clinica Chimica Acta. 181:317-323 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(89)90237-4 |
| Popis: | A sandwich-binding protein assay to determine the concentration of hyaluronic acid (HA) in body fluids has been developed. In this method, a hyaluronic acid binding protein (HABP) was adsorbed to the surface of a solid phase, and HA bound to HABP on the solid phase was detected by biotin-conjugated HABP. The method could assay HA levels within 6 hours using precoated microwells with HABP. HA could be determined in the range of 2-500 micrograms/l by this method using 50 microliters of serum. Within-run precision (CV) was 5.2-10.2%. The specificity of HABP to HA was confirmed by the elimination of the reaction with treatment by hyaluronidase digestion. Serum HA levels (median; range) of patients with rheumatoid arthritis (34; 2-187 micrograms/l) were shown to be higher than those with osteoarthritis (1; 1-21 micrograms/ml) and healthy controls (2; 1-8 micrograms/ml). No correlation between levels of HA and rheumatoid factor was found. HA was demonstrated to be a potential diagnostic marker for rheumatoid arthritis, and this HABP assay could be useful for determination of HA in clinical laboratory tests. |
| Databáze: | OpenAIRE |
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