Fungi in PAH-contaminated marine sediments: Cultivable diversity and tolerance capacity towards PAH

Autor: Robert Duran, Cristiana Cravo-Laureau, Joyce Álvarez-Barragán, Lukas Y. Wick
Přispěvatelé: Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), French/German bilateral program PHC-PROCOPE
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
Fusarium
Geologic Sediments
Fungal ITS
Polycyclic aromatic hydrocarbon
Peroxidase genes
010501 environmental sciences
Aquatic Science
Oceanography
01 natural sciences
chemistry.chemical_compound
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
polycyclic compounds
14. Life underwater
Internal transcribed spacer
Polycyclic Aromatic Hydrocarbons
0105 earth and related environmental sciences
Fluoranthene
chemistry.chemical_classification
PAH-uptake
biology
010604 marine biology & hydrobiology
Fungi
Alternaria
[CHIM.MATE]Chemical Sciences/Material chemistry
Phenanthrene
Ribosomal RNA
biology.organism_classification
Pollution
PAH-contamination
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
Biodegradation
Environmental
[CHIM.POLY]Chemical Sciences/Polymers
chemistry
Environmental chemistry
Pyrene
Zdroj: Marine Pollution Bulletin
Marine Pollution Bulletin, Elsevier, 2021, 164, pp.112082. ⟨10.1016/j.marpolbul.2021.112082⟩
ISSN: 0025-326X
DOI: 10.1016/j.marpolbul.2021.112082⟩
Popis: International audience; The cultivable fungal diversity from PAH-contaminated sediments was examined for the tolerance to polycyclic aromatic hydrocarbon (PAH). The 85 fungal strains, isolated in non-selective media, revealed a large diversity by ribosomal internal transcribed spacer (ITS) sequencing, even including possible new species. Most strains (64%) exhibited PAH-tolerance, indicating that sediments retain diverse cultivable PAH-tolerant fungi. The PAH-tolerance was linked neither to a specific taxon nor to the peroxidase genes (LiP, MnP and Lac). Examining the PAH-removal (degradation and/or sorption), Alternaria destruens F10.81 showed the best capacity with above 80% removal for phenanthrene, pyrene and fluoranthene, and around 65% for benzo[a]pyrene. A. destruens F10.81 internalized pyrene homogenously into the hyphae that contrasted with Fusarium pseudoygamai F5.76 in which PAH-vacuoles were observed but PAH removal was below 20%. Thus, our study paves the way for the exploitation of fungi in remediation strategies to mitigate the effect of PAH in coastal marine sediments.
Databáze: OpenAIRE
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