Effects of Calcipotriol and Clobetasol-17-Propionate on UVB-lrradiated Human Skin: An Immunohistochemical Study

Autor: Clementine G.E.M. Snijders, E.M.G.J. de Jong, C.J.M. van der Vleuten, P.C.M. van de Kerkhof
Rok vydání: 1996
Předmět:
Adult
Male
medicine.medical_specialty
Ultraviolet Rays
Physiology
Administration
Topical
Anti-Inflammatory Agents
Inflammation
Human skin
Dermatology
Pharmacology
Keratin 16
Ointments
chemistry.chemical_compound
Calcitriol
Psoriasis
Internal medicine
medicine
Humans
Glucocorticoids
Calcipotriol
Skin
Clobetasol
integumentary system
Epidermis (botany)
business.industry
Cell Differentiation
General Medicine
medicine.disease
Immunohistochemistry
Liganden van de steroid receptor superfamily
Ligands of the steröid receptor superfamily in dermatoses with abnormal epidermal growth and differentiation
Ki-67 Antigen
medicine.anatomical_structure
Endocrinology
chemistry
Erythema
Regression Analysis
Dermatologic Agents
Radiodermatitis
medicine.symptom
Clobetasol propionate
Keratinocyte
business
Cell Division
medicine.drug
Zdroj: Skin Pharmacology and Applied Skin Physiology, 9, pp. 355-365
Skin Pharmacology and Applied Skin Physiology, 9, 355-365
ISSN: 1660-5535
1660-5527
1422-2868
DOI: 10.1159/000211446
Popis: Corticosteroids and vitamin D3 analogues inhibit proliferation, enhance normal keratinisation and interfere with cutaneous inflammation in in vitro systems. Both treatments are effective in psoriasis, although several reports suggest that vitamin D3 is less effective in reducing the inflammatory changes compared to its potent effect on keratinocyte growth and differentiation. The aim of the present study was to compare and contrast the effects of the vitamin D3 analogue calcipotriol, clobetasol-17-propionate and a placebo on immunohistochemical markers for epidermal growth, keratinisation and inflammation induced by a standardised single challenge with ultraviolet B (UVB) radiation in normal human skin. Clobetasol proved to inhibit UVB-induced proliferation of epidermal cells, tenascin induction, keratin 16 induction and the accumulation of T lymphocytes and CD la-positive cells. Epidermal thinning due to clobetasol was also observed. No effect of clobetasol was shown on the enhanced terminal differentiation following UVB challenge. In contrast, calcipotriol reduced the member of transglutaminase-positive cells following UVB challenge but increased the thickness of the epidermis without a significant effect on other markers for keratinisation, epidermal proliferation and inflammation. The present study reconfirms the potent effect of topical corticosteroids on various aspects of UVB-challenged skin. In contrast, calcipotriol interfered especially with one differentiation pathway (transglutaminase) without modulation of other UVB-induced changes.
Databáze: OpenAIRE
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