Multicenter Comparative Evaluation of Six Commercial Systems and the National Committee for Clinical Laboratory Standards M27-A Broth Microdilution Method for Fluconazole Susceptibility Testing of Candida Species
| Autor: | M. G. Buscema, D. Tatò, F. Fanti, C. M C Belli, Piero Marone, Giulia Morace, Stefania Conti, C. Cavanna, Guido Fadda, Luciano Polonelli, Maria Teresa Montagna, Salvatore Massimo Oliveri, Francesco Bistoni, S. La Face, Christian Napoli, Lucia Pitzurra, Roberto Rigoli, M. M. Piccirillo, G. Amato, F. D'Alò, Lucio Romano, Luca Masucci, Silvia Covan, I. Mancuso |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Susceptibility testing Veterinary medicine Antifungal Agents Interlaboratory reproducibility C. albicans Microbial Sensitivity Tests Mycology Biology Comparative evaluation Microbiology medicine Humans Fluconazole Etest Candida Broth microdilution Candidiasis Reproducibility of Results equipment and supplies bacterial infections and mycoses Corpus albicans body regions Reagent Kits Diagnostic Laboratories medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 40:2953-2958 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.8.2953-2958.2002 |
| Popis: | Fluconazole susceptibility among 800 clinical Candida isolates (60% C. albicans ) and two control strains ( C. krusei ATCC 6258 and C. parapsilosis ATCC 22019) was tested with the NCCLS M27-A method (gold standard) and six commercial products (Candifast, disk, Etest, Fungitest, Integral System Yeasts, and Sensititre YeastOne). Results were classified as susceptible, susceptible-dose dependent, or resistant using M27-A breakpoints or, for Fungitest, Integral System Yeasts, and Candifast, as susceptible, intermediate, or resistant, according to the manufacturers' instructions. Concordance with NCCLS M27-A results was analyzed with the χ 2 test. Intra- and interlaboratory reproducibility was also evaluated. NCCLS M27-A (90.1%), Etest (93.1%), Sensititre YeastOne (93.1%), disk (96.7%), Fungitest (92.6%), Integral System Yeasts (40.6%), and Candifast (6.0%) classified the indicated percentages of C. albicans isolates as susceptible. Among non- C. albicans strains, the percentages of susceptible isolates were as follows: NCCLS M27-A, 74.0%; Etest, 83.8%; Sensititre YeastOne, 64.1%; disk, 60.6%; Fungitest, 76.6%; Integral System Yeasts, 28.3%; and Candifast, 27.4%. All methods except Candifast and Integral System Yeasts showed good agreement with NCCLS M27-A results for both C albicans and non- C. albicans isolates. Intralaboratory reproducibility was excellent for NCCLS M27-A, Etest, Sensititre YeastOne, disk, and Fungitest (88 to 91%). Similar results emerged from the interlaboratory reproducibility evaluation. Our findings indicate that some commercial methods can be useful for fluconazole susceptibility testing of clinical Candida isolates. Those characterized by a lack of medium standardization and/or objective interpretative criteria should be avoided. Particular caution is necessary when testing is being done for clinical and epidemiological purposes. |
| Databáze: | OpenAIRE |
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