Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging
Autor: | Wen Chyan, Ronald T. Raines |
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Rok vydání: | 2018 |
Předmět: |
chemistry.chemical_classification
Fluorophore 010405 organic chemistry Extramural Optical Imaging Chemical biology Nanotechnology General Medicine 010402 general chemistry 01 natural sciences Biochemistry Article Fluorescence Enzymes 0104 chemical sciences chemistry.chemical_compound Enzyme chemistry Cell Line Tumor Animals Humans Molecular Medicine Biological imaging Preclinical imaging Fluorescent Dyes |
Zdroj: | ACS Chemical Biology. 13:1810-1823 |
ISSN: | 1554-8937 1554-8929 |
Popis: | Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. Here, we review recent advances in enzyme-activated fluorogenic probes for biological imaging. We organize our survey by enzyme classification, with emphasis on fluorophore masking strategies, modes of enzymatic activation, and the breadth of current and future applications. Key challenges such as probe selectivity and spectroscopic requirements are described alongside of therapeutic, diagnostic, and theranostic opportunities. |
Databáze: | OpenAIRE |
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