Healthcare Workers Bioresource: Study outline and baseline characteristics of a prospective healthcare worker cohort to study immune protection and pathogenesis in COVID-19

Autor: Áine McKnight, Lee Howes, Gabriella Captur, Jessica Jones, Alicja Rapala, Steffen E. Petersen, Jessry Veerapen, Ivie Itua, Victor Jardim, Jessica Artico, Genine Sambile, Georgina L Baca, Vikas Kapil, Peter Griffiths, Celina Mfuko, George Joy, Ben O’Brien, Vineela Mandadapu, Lauren M Hickling, Mathew Robathan, Zoe Alldis, George Thornton, Kush Patel, Xose Couto-Parada, Rosalind Raine, Mashael Alfarih, Hakam Abbass, Art Tucker, Gemma A. Figtree, Wing-Yiu Jason Lee, Carmen Chan, Mahdad Noursadeghi, Andreas Seraphim, Jorge Couto de Sousa, Ruth Bowles, Melanie Figtree, Alex Boulter, James C. Moon, Theresa Wodehouse, Sophie Welch, Nicola Champion, Aroon D. Hingorani, Thomas A. Treibel, Michelle Sugimoto, Meleri Jones, Susana Palma, Dan Zahedi, Amanda Semper, Malcolm Finlay, Matt Hamblin, Hugh Montgomery, Tim Brooks, Marianna Fontana, Karen Feehan, Corinna Pade, Ruth M. Parker, Mohit Vijayakumar, Nasim Forooghi, Ntobeko A B Ntusi, João B Augusto, Charlotte Manisty, Natalie Bullock, Katia Menacho, Maudrian Burton, Lucinda Wynne, Teresa Cutino-Moguel, Rhodri H Davies, Brian Piniera, Oliver Mitchelmore, Anish N Bhuva, Keenan Dieobi-Anene, Mervyn Andiapen, Melanie Jensen, Amy Richards, Olivia V Bracken, Joseph M Gibbons
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Wellcome Open Research
Popis: Background: Most biomedical research has focused on sampling COVID-19 patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory. Methods: We conducted a prospective observational study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months. Results: Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies. Conclusions: This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/.
Databáze: OpenAIRE
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