Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Autor: Fariba Dehghanian, Lisa Winner, Leila Shabani, Fatemeh Raeisi Vanani, Mohammad R. Sabzalian
Rok vydání: 2020
Předmět:
Proteomics
0106 biological sciences
0301 basic medicine
Leaves
Antioxidant
medicine.medical_treatment
Plant Science
Iran
Biochemistry
01 natural sciences
Antioxidants
chemistry.chemical_compound
Plant Resistance to Abiotic Stress
Genotype
Photosynthesis
Amino Acids
Pollination
Abscisic acid
Plant Proteins
Cellular Stress Responses
chemistry.chemical_classification
Multidisciplinary
Ecology
biology
Organic Compounds
Plant Anatomy
Eukaryota
food and beverages
Plants
Glutathione
Droughts
Amino acid
Chemistry
Horticulture
Cell Processes
Plant Physiology
Physical Sciences
Medicine
Photorespiration
Research Article
Proline
Drought Adaptation
Science
Lolium perenne
03 medical and health sciences
Stress
Physiological
Plant-Environment Interactions
Lolium
medicine
Plant Defenses
Grasses
Physiology
Comparative
Plant Ecology
Ecology and Environmental Sciences
Organic Chemistry
Organisms
Chemical Compounds
Water
Biology and Life Sciences
Proteins
Cyclic Amino Acids
Cell Biology
Plant Pathology
biology.organism_classification
Plant Leaves
030104 developmental biology
chemistry
Ryegrass
Peptides
010606 plant biology & botany
Zdroj: PLoS ONE, Vol 15, Iss 6, p e0234317 (2020)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0234317
Popis: We investigated the physiological and proteomic changes in the leaves of three Lolium perenne genotypes, one Iranian putative self-pollinating genotype named S10 and two commercial genotypes of Vigor and Speedy, subjected to drought stress conditions. The results of this study indeed showed higher RWC (relative water content), SDW (shoot dry weight), proline, ABA (abscisic acid), nitrogen and amino acid contents, and antioxidant enzymes activities of S10 under drought stress in comparison with the two other genotypes. A total of 915 proteins were identified using liquid chromatography-mass spectrometry (LC/MS) analysis, and the number of differentially abundant proteins between normal and stress conditions was 467, 456, and 99 in Vigor, Speedy, and S10, respectively. Proteins involved in carbon and energy metabolism, photosynthesis, TCA cycle, redox, and transport categories were up-regulated in the two commercial genotypes. We also found that some protein inductions, including those involved in amino acid and ABA metabolisms, aquaporin, HSPs, photorespiration, and increases in the abundance of antioxidant enzymes, are essential responses of the two commercial genotypes to drought stress. In contrast, we observed only slight changes in the protein profile of the S10 genotype under drought stress. Higher homozygosity due to self-pollination in the genetic background of the S10 genotype may have led to a lower variation in response to drought stress conditions.
Databáze: OpenAIRE
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