Different Plasmodium falciparum clearance times in two Malian villages following artesunate monotherapy
| Autor: | Khalid B. Beshir, Ogobara K. Doumbo, Kadidia Haidara, Sekou Sissoko, Aliou Traore, José Pedro Gil, Demba Dembele, Kassim Sanogo, Nouhoum Diallo, Aboubecrine Sedhigh Haidara, Abdoulaye Djimde, Sekou Toure, Aminatou Kone, Cheick Papa Oumar Sangare, Issaka Sagara, Bakary Fofana, Aoua Coulibaly |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male medicine.medical_specialty Early signs 030106 microbiology Plasmodium falciparum Artesunate Parasitemia Mali Real-Time Polymerase Chain Reaction Gastroenterology Article lcsh:Infectious and parasitic diseases 03 medical and health sciences chemistry.chemical_compound Antimalarials 0302 clinical medicine Internal medicine parasitic diseases Medicine Parasite hosting Humans lcsh:RC109-216 030212 general & internal medicine Malaria Falciparum Child Parasite clearance Microscopy biology business.industry General Medicine biology.organism_classification medicine.disease Clearance time Malaria qPCR Infectious Diseases Blood smear chemistry Female business Artesunate monotherapy |
| Zdroj: | International Journal of Infectious Diseases International Journal of Infectious Diseases, Vol 95, Iss, Pp 399-405 (2020) |
| ISSN: | 1878-3511 1201-9712 |
| Popis: | Highlights • High prevalence of residual parasitemia at day 3 post-artesunate monotherapy treatment using qPCR while no parasites were detected by microscopy at the same timepoint. • A longer parasite clearance time observed in a Malian village. • Artesunate treatment is still efficacious on Plasmodium falciparum in Mali. Background Artemisinin resistance described as increased parasite clearance time (PCT) is rare in Africa. More sensitive methods such as qPCR might better characterize the clearance phenotype in sub-Saharan Africa. Methods PCT is explored in Mali using light microscopy and qPCR after artesunate for uncomplicated malaria. In two villages, patients were followed for 28 days. Blood smears and spots were collected respectively for microscopy and qPCR. Parasitemia slope half-life was calculated after microscopy. Patient residual parasitemia were measured by qPCR. Results Uncorrected adequate clinical and parasitological responses (ACPR) observed in Faladje and Bougoula-Hameau were 78% and 92%, respectively (p = 0.01). This reached 100% for both after molecular correction. Proportions of 24H microscopy positive patients in Faladje and Bougoula-Hameau were 97.2% and 72%, respectively (p |
| Databáze: | OpenAIRE |
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