Comparison of human embryomorphokinetic parameters in sequential or global culture media
| Autor: | Chloé Rousseau, Patricia Viard, Ségolène Veau, Hortense Drapier, Marc-Antoine Belaud-Rotureau, Célia Ravel, Guilhem Jouve, Cyril Bouche, Céline Pimentel, Nadia Kazdar, Florence Brugnon |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Laboratory Proficiency Testing Reproductive Techniques Assisted medicine.medical_treatment Embryonic Development Biology Time-Lapse Imaging Cell size Andrology Embryo Culture Techniques 03 medical and health sciences Young Adult 0302 clinical medicine Mitotic cell cycle Embryo Culture Technique medicine Humans Cells Cultured Cell Size 030219 obstetrics & reproductive medicine In vitro fertilisation Pronucleus Embryogenesis Embryo General Medicine Embryo Mammalian Sequential media Culture Media Embryo Research Kinetics 030104 developmental biology Female |
| Zdroj: | Annales de biologie clinique. 75(4) |
| ISSN: | 1950-6112 |
| Popis: | A prospective study on randomized patients was conducted to determine how morphokinetic parameters are altered in embryos grown in sequential versus global culture media. Eleven morphokinetic parameters of 160 single embryos transferred were analyzed by time lapse imaging involving two University-affiliated in vitro fertilization (IVF) centers. We found that the fading of the two pronuclei occurred earlier in global (22.56±2.15 hpi) versus sequential media (23.63±2.71 hpi; p=0.0297). Likewise, the first cleavage started earlier at 24.52±2.33 hpi vs 25.76±2.95 hpi (p=0.0158). Also, the first cytokinesis was shorter in global medium, lasting 18±10.2 minutes in global versus 36±37.8 minutes in sequential culture medium (p |
| Databáze: | OpenAIRE |
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