Proteomic analysis of halotolerant proteins under high and low salt stress in Dunaliella salina using two-dimensional differential in-gel electrophoresis
| Autor: | Jun-Fang Wu, Wang Xicheng, Jia Yanlong, Gao Lijie, Qiu Lele, Hui Chen, Chun-Lai Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 QH426-470 Proteomics Plant Genetics 01 natural sciences 03 medical and health sciences proteomics Genetics Extremophile Osmotic pressure Molecular Biology Amino acid synthesis chemistry.chemical_classification biology two-dimensional differential in-gel electrophoresis Metabolism biology.organism_classification lcsh:Genetics 030104 developmental biology chemistry Biochemistry Dunaliella salina Halotolerance halotolerant protein Function (biology) 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Biology Genetics and Molecular Biology, Vol 39, Iss 2, Pp 239-247 (2016) Genetics and Molecular Biology, Volume: 39, Issue: 2, Pages: 239-247, Published: 13 MAY 2016 Genetics and Molecular Biology v.39 n.2 2016 Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Iss 0 (2016) |
| ISSN: | 1678-4685 1415-4757 |
| Popis: | Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins under high (3 M NaCl) and low (0.75 M NaCl) salt concentrations. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and bioinformatics were used to identify and characterize the differences among proteins. 2D-DIGE analysis revealed 141 protein spots that were significantly differentially expressed between the two salinities. Twenty-four differentially expressed protein spots were successfully identified by MALDI-TOF/TOF MS, including proteins in the following important categories: molecular chaperones, proteins involved in photosynthesis, proteins involved in respiration and proteins involved in amino acid synthesis. Expression levels of these proteins changed in response to the stress conditions, which suggests that they may be involved in the maintenance of intracellular osmotic pressure, cellular stress responses, physiological changes in metabolism, continuation of photosynthetic activity and other aspects of salt stress. The findings of this study enhance our understanding of the function and mechanisms of various proteins in salt stress. |
| Databáze: | OpenAIRE |
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