Transcriptome Analysis of Porcine Granulosa Cells in Healthy and Atretic Follicles: Role of Steroidogenesis and Oxidative Stress
| Autor: | Chi Chiu Wang, Jian Tao, Katja J. Teerds, Shouquan Zhang, Zhenfang Wu, Kun Zhao, Tam Chit, Li Meng |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
antral follicular atresia endocrine system steroidogenesis Physiology Granulosa cell Clinical Biochemistry Steroid biosynthesis Biology medicine.disease_cause Biochemistry Article Andrology Transcriptome 03 medical and health sciences 0302 clinical medicine medicine oxidative stress Molecular Biology VLAG 030219 obstetrics & reproductive medicine Transcriptome profiles GCLM Follicular atresia Cell Biology 030104 developmental biology GCLC Steroidogenesis Oxidative stress Human and Animal Physiology Antral follicular atresia WIAS Fysiologie van Mens en Dier transcriptome profiles Immunostaining |
| Zdroj: | Antioxidants Volume 10 Issue 1 Antioxidants 10 (2021) 1 Antioxidants, 10(1), 1-17 |
| ISSN: | 2076-3921 |
| Popis: | One of the main causes of female infertility is a deregulated antral follicular atresia, a process of which the underlying molecular mechanisms are largely unknown. Our objective was therefore to characterize the complex transcriptome changes in porcine granulosa cells of healthy antral (HA) and advanced antral atretic (AA) follicles, using ELISA and RNA-Seq followed by qRT-PCR and immunohistochemistry. Granulosa cell RNA-Seq data revealed 2160 differentially expressed genes, 1483 with higher and 677 with lower mRNA concentrations in AA follicles. Bioinformatic analysis showed that the upregulated genes in AA follicles were highly enriched in inflammation and apoptosis processes, while the downregulated transcripts were mainly highlighted in the steroid biosynthesis pathway and response to oxidative stress processes including antioxidant genes (e.g., GSTA1, GCLC, GCLM, IDH1, GPX8) involved in the glutathione metabolism pathway and other redox-related genes (e.g., RRM2B, NDUFS4). These observations were confirmed by RT-qPCR and immunohistochemistry. Additionally, the granulosa cells of AA follicles express significantly stronger 8-OHdG immunostaining, a marker of oxidative DNA damage, implicating that oxidative stress may participate in follicular atresia. We hypothesize that the decrease in anti-apoptotic factors and steroid hormones coincides with increased oxidative stress markers and the expression of pro-apoptotic factors, all contributing to antral follicular atresia. |
| Databáze: | OpenAIRE |
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