Towards the Establishment of a Porcine Model to Study Human Amebiasis
| Autor: | Christelle Rossignol, Juliette Cognie, Timothée Bruel, Henri Salmon, Thibaut Larcher, Yan Cherel, François Meurens, Mario Delgado-Ortega, Sandrina Pereira Melo, Nancy Guillén, Fabienne Girard-Misguich, Pierre Sarradin, Patricia Berthon, Roseline Guibon |
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| Přispěvatelé: | Infection et inflammation chronique (2I), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Biologie Cellulaire du Parasitisme, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), UMR6175, Physiologie de la Reproduction et des Comportements, Centre National de la Recherche Scientifique (CNRS), Université Francois Rabelais [Tours], Institut Français du Cheval et de l'Equitation, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Développement et Pathologie du Tissu Musculaire (DPTM), Ecole Nationale Vétérinaire de Nantes-Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE - INRA UE 1277), This work was supported in part by the transversal research program INRA-Pasteur PTR-291: Development of an immuno-pathologic porcine model for intestinal human amebiasis and by the 'Conseil Regional du Centre' (France). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, Meurens, Francois |
| Rok vydání: | 2011 |
| Předmět: |
multidisciplinary sciences
Pathology Time Factors Swine [SDV]Life Sciences [q-bio] Gene Expression lcsh:Medicine Protozoology lésion Jejunum 0302 clinical medicine Intestinal mucosa Molecular Cell Biology animal modèle Gastrointestinal Infections disease models animal lcsh:Science 0303 health sciences Multidisciplinary Portal Vein Liver Diseases Dysentery Animal Models Amebiasis [SDV] Life Sciences [q-bio] jejunum parasitology Infectious Diseases medicine.anatomical_structure Dysentery Amebic Liver Abscess Amebic Medicine Female medicine.symptom Research Article Neglected Tropical Diseases science and technology medicine.medical_specialty Histology Clinical Research Design Colon Animal Types Immunology 030231 tropical medicine Gastroenterology and Hepatology Large Animals Biology Microbiology Injections amibiase entamoeba histolytica Enteritis Lesion 03 medical and health sciences Entamoeba histolytica Model Organisms intestin Gastrointestinal Surgery Parasitic Diseases medicine Animals Humans porcin Animal Models of Disease Trophozoites Amoebiasis Immunity to Infections maladie humaine 030304 developmental biology Inflammation lcsh:R Immunity medicine.disease biology.organism_classification Coculture Techniques Disease Models Animal Parastic Protozoans Clinical Immunology Surgery Veterinary Science lcsh:Q Liver abscess |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2011, 6 (12), ⟨10.1371/journal.pone.0028795⟩ PLoS ONE, Vol 6, Iss 12, p e28795 (2011) PLoS ONE, 2011, 6 (12), ⟨10.1371/journal.pone.0028795⟩ Plos One 12 (6) (2011) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0028795 |
| Popis: | BACKGROUND: Entamoeba histolytica is an important parasite of the human intestine. Its life cycle is monoxenous with two stages: (i) the trophozoite, growing in the intestine and (ii) the cyst corresponding to the dissemination stage. The trophozoite in the intestine can live as a commensal leading to asymptomatic infection or as a tissue invasive form producing mucosal ulcers and liver abscesses. There is no animal model mimicking the whole disease cycle. Most of the biological information on E. histolytica has been obtained from trophozoite adapted to axenic culture. The reproduction of intestinal amebiasis in an animal model is difficult while for liver amebiasis there are well-described rodent models. During this study, we worked on the assessment of pigs as a new potential model to study amebiasis. METHODOLOGY/PRINCIPAL FINDINGS: We first co-cultured trophozoites of E. histolytica with porcine colonic fragments and observed a disruption of the mucosal architecture. Then, we showed that outbred pigs can be used to reproduce some lesions associated with human amebiasis. A detailed analysis was performed using a washed closed-jejunal loops model. In loops inoculated with virulent amebas a severe acute ulcerative jejunitis was observed with large hemorrhagic lesions 14 days post-inoculation associated with the presence of the trophozoites in the depth of the mucosa in two out four animals. Furthermore, typical large sized hepatic abscesses were observed in the liver of one animal 7 days post-injection in the portal vein and the liver parenchyma. CONCLUSIONS: The pig model could help with simultaneously studying intestinal and extraintestinal lesion development. |
| Databáze: | OpenAIRE |
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