Efficacy of marine cyanobacterium Oxynema thaianum ALU PBC5 against multi drug resistant Gram negative pathogens
| Autor: | Nagarajan Padmini, Natesan Sivakumar, Pandian Prabakaran, Gopal Selvakumar, Antony Alex Kennedy Ajilda |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Klebsiella pneumoniae Antibiotics Microbial Sensitivity Tests medicine.disease_cause Cyanobacteria Applied Microbiology and Biotechnology High-performance liquid chromatography beta-Lactamases Microbiology 03 medical and health sciences Antibiotic resistance Enterobacteriaceae Drug Resistance Multiple Bacterial medicine Humans Escherichia coli 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry General Medicine biology.organism_classification Thin-layer chromatography Anti-Bacterial Agents Multiple drug resistance Chloroform Antibacterial activity Biotechnology |
| Zdroj: | Journal of applied microbiologyReferences. 130(1) |
| ISSN: | 1365-2672 |
| Popis: | Aim Emergence of extended antibiotic resistance among several human bacterial pathogens often leads to the failure of existing antibiotics to treat bacterial infections worldwide. Hence, the present study is aimed to explore antibacterial activity of marine cyanobacterium against MDR pathogens. Methods and results The cyanobacterial samples were collected and isolated from Thondi Palk Strait region. The isolate was subjected to polarity based solvent extraction and checked for their antibacterial activity against test bacterial pathogens. The active principles from chloroform extract of Oxynema thaianum (CEOT) were partially purified through thin layer chromatography (TLC). The active principle with highest activity was further characterized by FTIR, high performance liquid chromatography (HPLC) and gas chromatography mass spectrometry (GC-MS) analysis. Among the eight extracts tested, CEOT showed effective zone of clearance against ESBL producing Escherichia coli and Klebsiella pneumoniae in disc diffusion method. In TLC, all the purified five fractions were eluted and tested for their antibacterial activity against test pathogens. The third fraction showing maximum activity was subjected to HPLC analysis for checking its purity. In GC-MS analysis, 9-octadecenoic acid, methyl ester and hexadecanoic acid were identified as the major chemical compounds. Conclusion Hence, the present study was concluded that O. thaianum ALU PBC5 is a promising agent to treat ESBL producing MDR bacterial pathogens. Significance and impact of the study This is the pioneer study on screening and isolation of bioactive compounds from the marine cyanobacteria against MDR pathogens such as E. coli and K. pneumoniae. Here, 9-octadecenoic acid, methyl ester and hexadecanoic acid were identified as the major chemical compounds through TLC, FTIR, HPLC and GC-MS. From this screen, we identified the bioactive compounds against ESBL producing multidrug resistant pathogens such as E. coli and K. pneumoniae. |
| Databáze: | OpenAIRE |
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