SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
| Autor: | Xuepan Chen, Zhi-neng Zhu, Hanqing Zeng, Chao Cheng, Xingyuan Xiao, Wei Xiang, Jin Wang, Cameron Johnson, Shian Xing, Zongwei Wang, Youpeng Zhang, Zhaohui Zhu, Feng Wan, Sen Li |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Epithelial-Mesenchymal Transition lcsh:Medicine Vimentin Biology Metastasis medicine Humans Epithelial–mesenchymal transition Neoplasm Metastasis lcsh:Science Aged Multidisciplinary Bladder cancer Cell growth lcsh:R Mesenchymal stem cell Cell migration Matrix Attachment Region Binding Proteins Middle Aged Cell cycle medicine.disease Up-Regulation Urinary Bladder Neoplasms Gene Knockdown Techniques Disease Progression Cancer research biology.protein lcsh:Q Female Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 2, p e0117518 (2015) |
| ISSN: | 1932-6203 |
| Popis: | The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma (BTCC) and further elucidate if this effect works through an epithelial-mesenchymal transition (EMT) pathway. The expression of SATB1, E-cadherin (epithelial markers), vimentin (mesenchymal markers) in BTCC tissues and adjacent noncancerous tissues, as well as in two cell lines of bladder cancer were investigated. Whether the SATB1 expression is associated with clinicopathological factors or not was statistically analyzed. Cell invasion and migration, cell cycle, cell proliferation and apoptosis were evaluated in SATB1 knockdown and overexpressed cell lines. Our results showed that the expression of SATB1 was remarkably up-regulated both in BTCC tissues and in bladder cancer cell lines with high potential of metastasis. The results were also associated with EMT markers and poor prognosis of BTCC patients. Moreover, SATB1 induced EMT processes through downregulation of E-cadherin, upregulation of E-cadherin repressors (Snail, Slug and vimentin). SATB1 also promoted cell cycle progression, cell proliferation, cell invasion and cell migration, but did not alter cell survival. In conclusion, our results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes. Further, it may be a novel therapeutic target for aggressive bladder cancers. |
| Databáze: | OpenAIRE |
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