Detection of Glutathione by Glutathione-S-Transferase-Nanoconjugate Ensemble Electrochemical Device
| Autor: | Ujjwol Barman, Roy Paily, Namami Goswami, Siddhartha Sankar Ghosh, Gargi Mukhopadhyay |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Biomedical Engineering Analytical chemistry Pharmaceutical Science Medicine (miscellaneous) chemistry.chemical_element Nanoparticle Bioengineering Biosensing Techniques Nanoconjugates 02 engineering and technology Zinc Electrochemistry Electronic mail chemistry.chemical_compound Limit of Detection Electric Impedance 0202 electrical engineering electronic engineering information engineering Zeta potential Electrical and Electronic Engineering Glutathione Transferase Detection limit biology 020208 electrical & electronic engineering Reproducibility of Results Electrochemical Techniques Glutathione Enzymes Immobilized 021001 nanoscience & nanotechnology Computer Science Applications Glutathione S-transferase chemistry biology.protein Zinc Oxide 0210 nano-technology Biotechnology |
| Zdroj: | IEEE Transactions on NanoBioscience. 16:271-279 |
| ISSN: | 1558-2639 1536-1241 |
| Popis: | This paper reports a novel electrochemical method for detection of Glutathione (GSH) using Glutathione-S-Transferase (GST) - ZnO composite nanoparticles to investigate the prospects of the method for detection of cancer at an early stage. The purified GST enzyme was bound with ZnO nanoparticles by electrostatic interactions and the nanocomposite was dropcast on a silicon dioxide wafer. The GST functionalized deposited layer was then used as a chemiresistive channel to detect conjugation reaction between GSH and 1-Chloro-2, 4-Dinitrobenzene (CDNB). The zeta potential values of the ZnO nanoparticles and the GST were found to be 13.4 mV and–6.21 mV, respectively. Around 73.8% binding was observed between the enzyme and ZnO nanoparticles. ${I}$ – ${V}$ analysis of the chemiresistive channel showed an increase in conductivity of the channel due to conjugation reaction between GSH and CDNB as compared with that of GSH or CDNB alone. ${I}$ – ${V}$ characterization of the GST functionalized layer was performed at various concentrations of GSH and a sensitivity and limit of detection of 5.68 nA/ $\mu \text{M}$ and 41.9 nM were obtained, respectively. Thus from ${I}$ – ${V}$ analysis of the chemiresistivechannel, the detectionand quantification of GSH could be obtained. The kinetic parameters of both GST and nanoconjugate of ZnO nanoparticles andGSTwere determinedwith respect to its substrates, GSH and CDNB, using Michaelis–Mentenmodel. This novel approach of detection of GSH bymeans of ZnO nanoparticle and GST enzyme composite can be further analyzed for in vitro experiments, which will lead us to a new and efficient way of detecting certain types of cancers at an early stage. |
| Databáze: | OpenAIRE |
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