Characterization of hemicelluloses in sugarcane (Saccharum spp. hybrids) culm during xylogenesis
| Autor: | Na Yi, Huiling Li, Haoqiang Yang, Shuai Zhao, Jia-Xun Feng, Biao Zheng, Ai-Min Wu, Mirza Faisal Qaseem |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Arabinose
02 engineering and technology Xylose Biochemistry Saccharum 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Polysaccharides Structural Biology Botany Hemicellulose RNA-Seq Cellulose Molecular Biology 030304 developmental biology Hybrid chemistry.chemical_classification 0303 health sciences Plant Stems biology Chemistry Water Glycosidic bond General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Xylan Xylans 0210 nano-technology Bagasse |
| Zdroj: | International Journal of Biological Macromolecules. 165:1119-1128 |
| ISSN: | 0141-8130 |
| Popis: | Hemicelluloses are effective renewable biopolymers that can be used in many different industrial processes and preparations. In plants, the content of hemicellulose might change with different developmental stages and/or tissues. Thus, in here chemical and structural differences in hemicellulose isolated from the apical, middle and basal segments of sugarcane stem were characterized using chemical techniques. Further, difference in expression levels of genes related to synthesis of hemicelluloses from these three segments were studied by RNA-seq and qRT-PCR etc. The sugarcane hemicellulose backbone was xylose residues connected via β-1,4 glycosidic linkages which was further substituted with arabinose, acetyl and glucuronic acid side chains. Hemicellulose content was higher in the middle and basal segments with less backbone substitutions compared to apical segments. In terms of gene expression, hemicellulose synthesis and modification genes were intensely expressed in middle and basal segments. Taken together, our research describes differences in hemicellulose content and substitutions in sugarcane during xylogenesis, which will increase our knowledge for finding more refined use of sugarcane bagasse. |
| Databáze: | OpenAIRE |
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