Morroniside Protects Human Granulosa Cells against H2O2-Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways

Autor: Yucong Ma, Guimin Hao, Xiaohua Lin, Zhiming Zhao, Aimin Yang, Yucong Cao, Shuancheng Zhang, Lijie Fan, Jingran Geng, Yu Zhang, Jingwei Chen, Cuimiao Song, Ming He, Huilan Du
Rok vydání: 2022
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine. 2022:1-14
ISSN: 1741-4288
1741-427X
DOI: 10.1155/2022/8099724
Popis: Morroniside is the main ingredient of Cornus officinalis and has a variety of biological activities including antioxidative effects. Ovarian granulosa cells (GCs) are responsible for regulating the development and atresia of follicles, which are susceptible to oxidative stress. In this study, we determined whether morroniside can inhibit the oxidative stress of GCs induced by hydrogen peroxide (H2O2), leading to improved oocyte quality. The oxidative damage and apoptosis of ovarian GCs cultured in vitro were induced by the addition of H2O2. After pretreatment with morroniside, the levels of ROS, MDA, and 8-OHdG in ovarian GCs were significantly decreased. Morroniside significantly upregulated p-Nrf2 and promoted the nuclear translocation of Nrf2, which transcriptionally activated antioxidant SOD and NQO1. In addition, morroniside significantly regulated the levels of apoptosis-related proteins Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 via the p38 and JNK pathways. These results suggest that morroniside can reduce the oxidative damage and apoptosis of ovarian GCs induced by H2O2.
Databáze: OpenAIRE
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