Surveillance of Endoscopes: Comparison of Different Sampling Techniques

Autor: Tania Helleputte, Bruno Verhasselt, Lisa Florin, Isabel Leroux-Roels, Jerina Boelens, Thomas Vanzieleghem, Lien Cattoir, Martine De Vos
Rok vydání: 2017
Předmět:
Microbiology (medical)
BIOFILMS
Microbiological culture
Endoscope
Epidemiology
medicine.medical_treatment
Acceptance rate
CULTURES
Sodium Chloride
law.invention
Hospitals
University
03 medical and health sciences
0302 clinical medicine
Adenosine Triphosphate
Belgium
law
INFECTION
Gram-Negative Bacteria
Medicine and Health Sciences
medicine
Escherichia coli
Staphylococcus epidermidis
QUALITY
Humans
030212 general & internal medicine
Saline
Physiological saline
ADENOSINE-TRIPHOSPHATE
Endoscopes
Bacteriological Techniques
medicine.diagnostic_test
business.industry
Biology and Life Sciences
Brush
FLEXIBLE GASTROINTESTINAL ENDOSCOPES
Original Articles
University hospital
Endoscopy
Disinfection
Klebsiella pneumoniae
Infectious Diseases
GUIDELINE
Biofilms
Pseudomonas aeruginosa
Equipment Contamination
030211 gastroenterology & hepatology
DISINFECTION
business
Biomedical engineering
Zdroj: Infection Control and Hospital Epidemiology
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
ISSN: 1559-6834
0899-823X
Popis: OBJECTIVETo compare different techniques of endoscope sampling to assess residual bacterial contamination.DESIGNDiagnostic study.SETTINGThe endoscopy unit of an 1,100-bed university hospital performing ~13,000 endoscopic procedures annually.METHODSIn total, 4 sampling techniques, combining flushing fluid with or without a commercial endoscope brush, were compared in an endoscope model. Based on these results, sterile physiological saline flushing with or without PULL THRU brush was selected for evaluation on 40 flexible endoscopes by adenosine triphosphate (ATP) measurement and bacterial culture. Acceptance criteria from the French National guideline (RESULTSOn biofilm-coated PTFE tubes, physiological saline in combination with a PULL THRU brush generated higher mean ATP values (2,579 relative light units [RLU]) compared with saline alone (1,436 RLU; P=.047). In the endoscope samples, culture yield using saline plus the PULL THRU (mean, 43 CFU; range, 1–400 CFU) was significantly higher than that of saline alone (mean, 17 CFU; range, 0–500 CFU; PP=.001).CONCLUSIONPhysiological saline flushing combined with PULL THRU brush to sample endoscopes generated higher ATP values and increased the yield of microbial surveillance culture. Consequently, the acceptance rate of endoscopes based on a defined CFU limit was significantly lower when the saline+PULL THRU method was used instead of saline alone.Infect Control Hosp Epidemiol 2017;38:1062–1069
Databáze: OpenAIRE
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