New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
| Autor: | Marina Muñoz, Dora I. Ríos-Chaparro, Giovanny Herrera, Sara C. Soto-De Leon, Claudia Birchenall, Darío Pinilla, Juan M. Pardo-Oviedo, Diego F. Josa, Manuel A. Patarroyo, Juan D. Ramírez |
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| Přispěvatelé: | Grupo de Investigaciones Microbiológicas - UR (GIMUR) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Diarrhea
0301 basic medicine Microbiology (medical) Real Time Polymerase Chain Reaction 030106 microbiology Health Care Facility lcsh:QR1-502 Gene Expression Clostridium difficile toxin A Clostridium difficile toxin B Clostridium Difficile Infection Colombia Biology Marcador Molecular Disease Control Microbiology Article lcsh:Microbiology Molecular Marker 03 medical and health sciences Dna Extraction Genetic Marker medicine Controlled Study Dna imprimación Clostridium Difficile Toxin A Clostridium Difficile Toxin B Original Research Rna 16S Feces analysis toxins Primer Dna Clostridium difficile In vitro culture Enfermedades DNA extraction In Vitro Study qPCR Real-time polymerase chain reaction PCR Practice Guideline Antibiotic-associated diarrhea medicine.symptom Feces Analysis Human |
| Zdroj: | Frontiers in Microbiology, Vol 9 (2018) Frontiers in Microbiology Allegranzi, B., Bagheri Nejad, S., Combescure, C., Graafmans, W., Attar, H., Donaldson, L., Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis (2011) Lancet, 377, pp. 228-241 Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| DOI: | 10.3389/fmicb.2018.00074/full |
| Popis: | Clostridium difficile (CD) produces antibiotic associated diarrhea and leads to a broad range of diseases. The source of CD infection (CDI) acquisition and toxigenic profile are factors determining the impact of CD. This study aimed at detecting healthcare facility onset- (HCFO) and community-onset (CO) CDI and describing their toxigenic profiles in Bogotá, Colombia. A total of 217 fecal samples from patients suffering diarrhea were simultaneously submitted to two CDI detection strategies: (i) in vitro culture using selective chromogenic medium (SCM; chromID, bioMérieux), followed verification by colony screening (VCS), and (ii) molecular detection targeting constitutive genes, using two conventional PCR tests (conv. PCR) (conv.16S y conv.gdh) and a quantitative test (qPCR.16s). The CD toxigenic profile identified by any molecular test was described using 6 tests independently for describing PaLoc and CdtLoc organization. High overall CDI frequencies were found by both SCM (52.1%) and conv. PCR (45.6% for conv.16S and 42.4% for conv.gdh), compared to reductions of up to half the frequency by VCS (27.2%) or qPCR.16S (22.6%). Infection frequencies were higher for SCM and conv.16S regarding HCFO but greater for CO concerning conv.gdh, such differences being statistically significant. Heterogeneous toxigenic profiles were found, including amplification with lok1/3 primers simultaneously with other PaLoc markers (tcdA, tcdB or tcdC). These findings correspond the first report regarding the differential detection of CDI using in vitro culture and molecular detection tests in Colombia, the circulation of CD having heterogeneous toxigenic profiles and molecular arrays which could affect the impact of CDI epidemiology. © 2018 Muñoz, Ríos-Chaparro, Herrera, Soto-De Leon, Birchenall, Pinilla, Pardo-Oviedo, Josa, Patarroyo and Ramírez. |
| Databáze: | OpenAIRE |
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