In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption
| Autor: | Cláudio Cabral Campello, Laritza Ferreira de Lima, D.M. Magalhães-Padilha, G.M. Silva, L.B.R. Silveira, L.A. Vieira, I.R. Brito, José Ricardo de Figueiredo, A.P.R. Rodrigues, A.D. Sales, Matthew B. Wheeler, F.L.N. Aguiar, Ana Beatriz Graça Duarte, Valdevane Rocha Araújo, Benner Geraldo Alves, E.G. Lo Turco |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty medicine.medical_treatment Biology law.invention 03 medical and health sciences Ovarian Follicle Food Animals Meiosis In vivo law Internal medicine medicine Animals Insulin Small Animals Germinal vesicle urogenital system Equine Goats Petri dish Oocyte Coculture Techniques In vitro Culture Media In Vitro Oocyte Maturation Techniques 030104 developmental biology medicine.anatomical_structure Endocrinology Oocytes Female Animal Science and Zoology Follicle Stimulating Hormone In vitro growth |
| Zdroj: | Theriogenology. 90:32-41 |
| ISSN: | 0093-691X |
| Popis: | The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicles and (2) to analyze the efficiency of two IVM media and maturation culture systems (with or without coculture with in vivo grown oocytes) on the meiosis resumption. Secondary follicles were cultured for 18 days in a basic medium supplemented with low- or high-insulin concentration alone or with a fixed FSH concentration or with increasing FSH concentrations. Oocytes grown in vivo or in vitro were matured alone or cocultured. The high-insulin concentration associated with fixed FSH treatment had higher meiotic resumption rate (P < 0.05) and was the only treatment capable of producing oocytes in metaphase II. The rates of germinal vesicle, germinal vesicle breakdown, metaphase I, metaphase II (MII), meiotic resumption, and oocyte diameter were similar between the maturation media. In conclusion, a basic medium supplemented with 10-μg/mL insulin and 100-μg/mL FSH throughout the culture period improved meiotic resumption rate and produced MII oocytes from caprine preantral follicles cultured in vitro. The MII rate was similar between in vivo and in vitro grown oocytes ≥110 μm. |
| Databáze: | OpenAIRE |
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