Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode
| Autor: | Percio A. M. Farias, Arnaldo Aguiar Castro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty stripping voltammetry Supporting electrolyte lcsh:Analytical chemistry chemistry.chemical_element Biology Biochemistry Analytical Chemistry chemistry.chemical_compound Adsorptive stripping voltammetry ssDNA medicine Hypoxanthine Original Research Detection limit lcsh:QD71-142 Dropping mercury electrode Xanthine Mercury (element) ATP Medical Laboratory Technology chemistry hypoxanthine copper Uric acid xanthine determination mercury electrode Nuclear chemistry |
| Zdroj: | Analytical Chemistry Insights, Vol 2014, Iss 9, Pp 49-55 (2014) Analytical Chemistry Insights Analytical Chemistry Insights, Vol 9 (2014) |
| ISSN: | 1177-3901 |
| Popis: | A stripping method for the determination of xanthine in the presence of hypoxanthine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10(-3) mol L(-1) NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and -0.50 V for hypoxanthine-copper, and a linear scan rate of 200 mV second(-1). The response of xanthine is linear over the concentration ranges of 20-140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10(-10) mol L(-1)). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of xanthine associated with hypoxanthine, uric acid, nitrogenated bases, ATP, and ssDNA. |
| Databáze: | OpenAIRE |
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