Evidence for binding of extrachromosomal DNA sequences to nuclear matrix proteins in multidrug-resistant KB-V1 cells
| Autor: | Axel-R. Hanauske, Daniel D. Von Hoff, Franz Thiebaut |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Electrophoresis
Biophysics Drug Resistance Biology Sodium Chloride Biochemistry Plasmid Cell Line chemistry.chemical_compound Structural Biology Extrachromosomal DNA Gene expression Genetics Nuclear Matrix Trypsin Nuclear protein Molecular Biology Gene In Situ Hybridization Serine Endopeptidases Nuclear Proteins Cell Biology DNA Nuclear matrix Molecular biology Nuclear DNA chemistry Microscopy Fluorescence Episome KB-V1 cell Endopeptidase K Plasmids |
| Zdroj: | FEBS letters. 319(1-2) |
| ISSN: | 0014-5793 |
| Popis: | Multidrug-resistant KB-V1 cells carry amplified mdrl gene sequences located in an extrachromosomal compartment (on episomes). Since episomes do not contain centromeric or telomeric sequences it is unclear whether they are able to bind to nuclear matrix proteins that may regulate episomal gene expression. Using high salt treatments followed by in situ hybridization and dot blot analyses we found evidence for direct binding of episomal DNA to nuclear matrix proteins. This binding could only be reversed after incubation with trypsin or proteinase K as determined by contour-clamped homogeneous electric field (CHEF) electrophoresis. Our findings are consistent with the concept that circular extrachromosomal DNA may not only reintegrate into nuclear DNA but may also be subject to functional control by regulatory proteins within the nuclear matrix. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text