Bioinspired Liposomes for Oral Delivery of Colistin to Combat Intracellular Infections bySalmonella enterica
Autor: | Sarah Gordon, Markus Bischoff, Marcus Koch, Sara Menina, Mohamed Ashraf M. Kamal, Janina Eisenbeis, Claus-Michael Lehr, Brigitta Loretz |
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Přispěvatelé: | HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany. |
Rok vydání: | 2019 |
Předmět: |
Staphylococcus aureus
RM medicine.drug_class Antibiotics Intracellular Space Biomedical Engineering Administration Oral Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences extracellular adherence proteins Microbiology Biomaterials Bacterial Proteins Biomimetics medicine Extracellular Humans Host cell membrane Liposome Microbial Viability biology Colistin Chemistry Intracellular parasite RNA-Binding Proteins Salmonella enterica Epithelial Cells bacterial invasion bacteriomimetic nanocarriers 021001 nanoscience & nanotechnology biology.organism_classification Eap 0104 chemical sciences simulated intestinal fluids Liposomes Salmonella Infections Caco-2 Cells 0210 nano-technology Intracellular medicine.drug |
Zdroj: | Advanced healthcare materials |
ISSN: | 2192-2659 2192-2640 |
Popis: | Bacterial invasion into eukaryotic cells and the establishment of intracellular infection has proven to be an effective means of resisting antibiotic action, as anti-infective agents commonly exhibit a poor permeability across the host cell membrane. Encapsulation of anti-infectives into nanoscaled delivery systems, such as liposomes, is shown to result in an enhancement of intracellular delivery. The aim of the current work is, therefore, to formulate colistin, a poorly permeable anti-infective, into liposomes suitable for oral delivery, and to functionalize these carriers with a bacteria-derived invasive moiety to enhance their intracellular delivery. Different combinations of phospholipids and cholesterol are explored to optimize liposomal drug encapsulation and stability in biorelevant media. These liposomes are then surface-functionalized with extracellular adherence protein (Eap), derived from Staphylococcus aureus. Treatment of HEp-2 and Caco-2 cells infected with Salmonella enterica using colistin-containing, Eap-functionalized liposomes resulted in a significant reduction of intracellular bacteria, in comparison to treatment with nonfunctionalized liposomes as well as colistin alone. This indicates that such bio-invasive carriers are able to facilitate intracellular delivery of colistin, as necessary for intracellular anti-infective activity. The developed Eap-functionalized liposomes, therefore, present a promising strategy for improving the therapy of intracellular infections. |
Databáze: | OpenAIRE |
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