Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection
| Autor: | Alex F. Lainson, Lisa Imrie, Eleanor Watson, Kevin McLean, M. Pilar Alberdi, David Smith, Megan E. Porter, Neil F. Inglis, Erin D. T. Manson |
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| Přispěvatelé: | Scottish Government's Rural and Environment Science and Analytical Services, Biotechnology and Biological Sciences Research Council (UK) |
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Gram-negative bacteria Swine Lawsonia Bacteria Microbiology Genome Lawsonia intracellularis Proliferative enteropathy Bacterial Proteins Tandem Mass Spectrometry Protein biosynthesis Shotgun proteomics Animals Gene Swine Diseases General Veterinary biology Base Sequence Surface proteins Computational Biology Membrane Proteins General Medicine biology.organism_classification Blot Desulfovibrionaceae Infections Intestinal Diseases Serology Host-Pathogen Interactions Bacterial outer membrane Genome Bacterial |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Lawsonia intracellularis is the aetiological agent of the commercially significant porcine disease, proliferative enteropathy. Current understanding of host-pathogen interaction is limited due to the fastidious microaerophilic obligate intracellular nature of the bacterium. In the present study, expression of bacterial proteins during infection was investigated using a mass spectrometry approach. LC-ESI-MS/MS analysis of two isolates of L. intracellularis from heavily-infected epithelial cell cultures and database mining using fully annotated L. intracellularis genome sequences identified 19 proteins. According to the Clusters of Orthologous Groups (COG) functional classification, proteins were identified with roles in cell metabolism, protein synthesis and oxidative stress protection; seven proteins with putative or unknown function were also identified. Detailed bioinformatic analyses of five uncharacterised proteins, which were expressed by both isolates, identified domains and motifs common to other outer membrane-associated proteins with important roles in pathogenesis including adherence and invasion. Analysis of recombinant proteins on Western blots using immune sera from L. intracellularis-infected pigs identified two proteins, LI0841 and LI0902 as antigenic. The detection of five outer membrane proteins expressed during infection, including two antigenic proteins, demonstrates the potential of this approach to interrogate L. intracellularis host-pathogen interactions and identify novel targets which may be exploited in disease control. This work was supported by BBSRC research grant BB/C510532/1. Moredun Research Institute receives funding from Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS). |
| Databáze: | OpenAIRE |
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