An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections
| Autor: | Jaap Goudsmit, John J. Schalken, Vera J. P. Teeuwsen, Guido van der Groen, Albert D. M. E. Osterhaus, Ruud van de Akker |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
medicine.diagnostic_test
Serial dilution medicine.drug_class Immunology Antibodies Monoclonal virus diseases HIV Infections Biology Monoclonal antibody Virology Virus HIV Envelope Protein gp41 Serology Immunoenzyme Techniques Infectious Diseases Evaluation Studies as Topic Immunoassay Monoclonal medicine biology.protein HIV-1 Immunology and Allergy Humans Viral disease Antibody |
| Zdroj: | AIDS (London, England), 5(7), 893-896. Lippincott Williams and Wilkins |
| ISSN: | 0269-9370 |
| Popis: | An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV(ANI70) infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|