A willow UDP-glycosyltransferase involved in salicinoid biosynthesis
| Autor: | Satish Kulasekaran, Femke de Jong, Gianluca Ruvo, Sergio Cerezo-Medina, Amelie Rendour, Steven J. Hanley, Charlotte Lomax, Michael H. Beale, Claudia Harflett, Jane L. Ward |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science Genetically modified crops 01 natural sciences Uridine Diphosphate 03 medical and health sciences chemistry.chemical_compound Metabolomics Glucoside Salicin Biosynthesis Salicaceae Glycosyltransferase Glycosides Tremulacin chemistry.chemical_classification biology fungi Salicinoid Glycosyltransferases Glycoside food and beverages Salix Plants Genetically Modified biology.organism_classification Salicortin Salicyl-7-benzoate Populus 030104 developmental biology chemistry Biochemistry biology.protein UGT Poplar 010606 plant biology & botany |
| Popis: | The salicinoids are phenolic glycosides that are characteristic secondary metabolites of the Salicaceae, particularly willows and poplars. Despite the well-known pharmacology of salicin, that led to the development of aspirin >100 years ago, the biosynthetic pathways leading to salicinoids have yet to be defined. Here, we describe the identification, cloning, and biochemical characterization of SpUGT71L2 and SpUGT71L3—isozymic glycosyltransferases from Salix purpurea—that function in the glucosylation of ortho-substituted phenols. The best substrate in vitro was salicyl-7-benzoate. Its product, salicyl-7-benzoate glucoside, was shown to be endogenous in poplar and willow. Together they are inferred to be early intermediates in the biosynthesis of salicortin and related metabolites in planta. The role of this UDP-glycosyltransferase was confirmed via the metabolomic analysis of transgenic plants produced by RNAi knockdown of the poplar orthologue (UGT71L1) in the hybrid clone Populus tremula×P. alba, INRA 717-1B4. |
| Databáze: | OpenAIRE |
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