Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin

Autor:	Nathalie Bock, Margaux Vigata, Dietmar W. Hutmacher, Christoph Meinert, Silvia Cometta, Cathal D O'Connell
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	food.ingredient Polymers and Plastics medicine.drug_class Antibiotics Cefazolin Organic chemistry 02 engineering and technology Antibacterial efficacy Cefazolin Dose 010402 general chemistry 01 natural sciences Gelatin Article food QD241-441 surgery site infection medicine localized antibiotic therapy gelatin methacryloyl Chemistry General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences 3. Good health Self-healing hydrogels Drug delivery drug delivery cefazolin Delivery system 0210 nano-technology Biomedical engineering medicine.drug
Zdroj:	Polymers Polymers, Vol 13, Iss 3960, p 3960 (2021) Volume 13 Issue 22
ISSN:	2073-4360
Popis:	The tuneability of hydrogels renders them promising candidates for local drug delivery to prevent and treat local surgical site infection (SSI) while avoiding the systemic side-effects of intravenous antibiotic injections. Here, we present a newly developed gelatin methacryloyl (GelMA)-based hydrogel drug delivery system (GelMA-DDS) to locally deliver the broad-spectrum antibiotic cefazolin for SSI prophylaxis and treatment. Antibiotic doses from 3 µg to 90 µg were loaded in photocrosslinked GelMA hydrogel discs with 5 to 15% w/v polymer concentration and drug encapsulation efficiencies, mechanical properties, crosslinking and release kinetics, as well as bacterial growth inhibition were assessed. Our results demonstrate that all GelMA groups supported excellent drug encapsulation efficiencies of up to 99%. Mechanical properties of the GelMA-DDS were highly tuneable and unaffected by the loading of small to medium doses of cefazolin. The diffusive and the proteolytic in vitro drug delivery of all investigated cefazolin doses was characterized by a burst release, and the delivered cefazolin amount was directly proportional to the encapsulated dose. Accelerated enzymatic degradation of the GelMA-DDS followed zero-order kinetics and was dependent on both the cefazolin dose and GelMA concentration (3–13 h). Finally, we demonstrate that cefazolin delivered from GelMA induced a dose-dependent antibacterial efficacy against S. aureus, in both a broth and a diffusive assay. The cefazolin-loaded GelMA-DDS presented here provides a highly tuneable and easy-to-use local delivery system for the prophylaxis and treatment of SSI.
Databáze:	OpenAIRE
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