Impact of Polymer Tether Length on Multiple Ligand-Receptor Bond Formation

Autor:	Nasreen Mullah, Joyce Wong, Tonya L. Kuhl, Claus Jeppesen, Jacob N. Israelachvili, Samuel Zalipsky, Carlos M. Marques
Rok vydání:	2001
Předmět:	Chemical Phenomena Polymers Protein Conformation Surface Properties Stereochemistry Kinetics Binding energy Monte Carlo method Biotin Ligands Polyethylene Glycols Diffusion Cell adhesion chemistry.chemical_classification Multidisciplinary Chemistry Physical Ligand Chemistry Surface force Adhesion Polymer Chemical physics Thermodynamics Streptavidin Monte Carlo Method Mathematics
Zdroj:	Science. 293:465-468
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.293.5529.465
Popis:	The promoters of cell adhesion are ligands, which are often attached to flexible tethers that bind to surface receptors on adjacent cells. Using a combination of Monte Carlo simulations, diffusion reaction theory, and direct experiments (surface force measurements) of the biotin-streptavidin system, we have quantified polymer chain dynamics and the kinetics and spatial range of tethered ligand-receptor binding. The results show that the efficiency of strong binding does not depend solely on the molecular architecture or binding energy of the receptor-ligand pair, nor on the equilibrium configuration of the polymer tether, but rather on its “rare” extended conformations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::842eec57c51a33fb57980045c87cdd7f https://doi.org/10.1126/science.293.5529.465 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.