Quantitative analysis of the composition of mixtures of one-chain and two-chain tissue-type plasminogen activator with a spectrophotometric method
| Autor: | G.T.G. Chang Gaubius, J.H. Verheijen, Y.F. de Jong |
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| Rok vydání: | 1985 |
| Předmět: |
Chromatography
Chemistry Protein Conformation Hematology Tissue plasminogen activator Substrate Specificity Kinetics Plasminogen Activators Activity measurements Chain (algebraic topology) Spectrophotometry medicine Tissue type Humans Composition (visual arts) Quantitative analysis (chemistry) Plasminogen activator Oligopeptides medicine.drug |
| Zdroj: | Thrombosis research. 39(3) |
| ISSN: | 0049-3848 |
| Popis: | Tissue-type plasminogen activator (t-PA) exists in two active forms, one-chain t-PA and two-chain t-PA. These two forms differ in their kinetic constants for certain substrates. An assay has been devised to determine the composition of unknown mixtures of both t-PA forms, using the activity measurement on two different substrates. The best results are obtained using plasminogen and less than Glu-Pro-Arg-pNA as substrates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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